Western Blot (WB)
(Fig-1: Western blot analysis of DNMT2. Anti-DNMTc2 antibody (Clone: ABM1H70) was used at 4 ug/ml on Jurkat and mouse embryonic liver lysates.)
Mouse anti-Mouse, Human DNMT2 Monoclonal Antibody | anti-DNMT2 antibody
Monoclonal Antibody to DNMT2 (Clone: ABM1H70)
Gene Names
TRDMT1; DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
Reactivity
Mouse, Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
PurifiedProtein G Chromatography
Synonyms
DNMT2; Monoclonal Antibody; Monoclonal Antibody to DNMT2 (Clone: ABM1H70); HCG23994; isoform CRA_a; cDNA FLJ76302; Highly similar to Homo sapiens DNA (cytosine-5-)-methyltransferase 2 (DNMT2); tRNA aspartic acid methyltransferase 1 variant 3; anti-DNMT2 antibody
Host
Mouse
Reactivity
Mouse, Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
ABM1H70
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
391
Applicable Applications for anti-DNMT2 antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
WB: 4-6 ug/ml
Flow Cytometry: 0.5-1 ug/10^6
Flow Cytometry: 0.5-1 ug/10^6
Immunogen Information
A partial length recombinant protein from DNMT2 was used as the immunogen for this antibody.
Antigen
DNMT2
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig-1: Western blot analysis of DNMT2. Anti-DNMTc2 antibody (Clone: ABM1H70) was used at 4 ug/ml on Jurkat and mouse embryonic liver lysates.)
Western Blot (WB)
(Fig-1: Western blot analysis of DNMT2. Anti-DNMTc2 antibody (Clone: ABM1H70) was used at 4 ug/ml on Jurkat and mouse embryonic liver lysates.)
Flow Cytometry (FC/FACS)
(Fig-2: Intracellular Flow analysis of DNMT2 antibody in Hela cells using 0.5 ug/ 10^6 cells of anti-DNMT2 antibody (ABM1H70). Green represents isotype control; red represents anti-DNMT 2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Flow Cytometry (FC/FACS)
(Fig-2: Intracellular Flow analysis of DNMT2 antibody in Hela cells using 0.5 ug/ 10^6 cells of anti-DNMT2 antibody (ABM1H70). Green represents isotype control; red represents anti-DNMT 2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Related Product Information for anti-DNMT2 antibody
DNMT2, also known as tRNA-aspartic acid methyltransferase 1 (TRDMT1), is highly conserved cytosine-C5 methyltransferase that introduces the C38 methylation of tRNAAsp in many species, including lower eukaryotes, plants, insects, and humans. DNMT2 contains all 10 sequence motifs that are conserved among m5C MTases, including the consensus S-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide. It is a relatively small protein of 391 amino acids and lacks the large N-terminal domains present in the DNMT1 and DNMT3 families. DNMT2 is ubiquitously expressed with multiple mRNA species in most human and mouse adult tissues. It is variably expressed in human cancer cell lines.
Product Categories/Family for anti-DNMT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,597 Da
NCBI Official Full Name
DNMT2 protein
NCBI Official Synonym Full Names
tRNA aspartic acid methyltransferase 1
NCBI Official Symbol
TRDMT1
NCBI Official Synonym Symbols
DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
NCBI Protein Information
tRNA (cytosine(38)-C(5))-methyltransferase
UniProt Protein Name
DNMT2 protein
UniProt Gene Name
DNMT2
NCBI Description
This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]
Research Articles on DNMT2
Similar Products
Product Notes
The DNMT2 dnmt2 (Catalog #AAA668323) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to DNMT2 (Clone: ABM1H70) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNMT2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). WB: 4-6 ug/ml Flow Cytometry: 0.5-1 ug/10^6. Researchers should empirically determine the suitability of the DNMT2 dnmt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNMT2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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