Immunofluorescence (IF)
(Immunofluorescence staining of TRDMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TRDMT1 polyclonal antibody (dilution ratio 1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)
Rabbit anti-Human DNMT2 Polyclonal Antibody | anti-TRDMT1 antibody
Anti-DNMT2 Antibody, Rabbit Polyclonal
Gene Names
TRDMT1; DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
DNMT2; Polyclonal Antibody; Anti-DNMT2 Antibody; Rabbit Polyclonal; DNMT2/TRDMT1 Antibody; Rabbit PAb; Antigen Affinity Purified; tRNA aspartic acid methyltransferase 1; Anti-DMNT2 Antibody; Anti-MHSAIIP Antibody; Anti-PUMET Antibody; Anti-RNMT1 Antibody; anti-TRDMT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DNMT2/TRDMT1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TRDMT1 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:1500-1:50,000
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human DNMT2/TRDMT1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DNMT2/TRDMT1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of TRDMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TRDMT1 polyclonal antibody (dilution ratio 1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of TRDMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TRDMT1 polyclonal antibody (dilution ratio 1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)
Related Product Information for anti-TRDMT1 antibody
DNMT2, also known as tRNA (cytosine-5-)-methyltransferase, DNA methyltransferase homolog HsaIIP, and TRDMT1, is a member of the DNA methyltransferase family of enzymes. DNMT2 enzymes have been widely conserved during evolution and contain all of the signature motifs of DNA (cytosine-5)-methyltransferases. It contains all 10 sequence motifs that are conserved among m(5)C MTases, including the consensus S:-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide, and its structure is very similar to prokaryotic DNA methyltransferases. DNMT2 has close homologs in plants, insects and Schizosaccharomyces pombe, but no related sequence can be found in the genomes of Saccharomyces cerevisiae or Caenorhabditis elegans. While the biological function of DNMT2 is not yet known, the strong binding to DNA suggests that DNMT2 may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. However, the DNA methyltransferase activity of these proteins is comparatively weak and their biochemical and functional properties remain enigmatic. Recent evidence now shows that Dnmt2 has a novel tRNA methyltransferase activity, raising the possibility that the biological roles of these proteins might be broader than previously thought.
Product Categories/Family for anti-TRDMT1 antibody
References
Dong A, et al. (2001) Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNA. Nucleic Acids Res. 29(2): 439-48.Hermann A, et al. (2003) The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity. J Biol Chem. 278(34): 31717-21.Jeltsch A, et al. (2006) Two substrates are better than one: dual specificities for Dnmt2 methyltransferases. Trends Biochem Sci. 31(6): 306-8.Schaefer M, et al. (2010) Solving the Dnmt2 enigma. Chromosoma. 119(1): 35-40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,087 Da
NCBI Official Full Name
tRNA (cytosine(38)-C(5))-methyltransferase
NCBI Official Synonym Full Names
tRNA aspartic acid methyltransferase 1
NCBI Official Symbol
TRDMT1
NCBI Official Synonym Symbols
DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
NCBI Protein Information
tRNA (cytosine(38)-C(5))-methyltransferase; tRNA (cytosine(38)-C(5))-methyltransferase; DNA (cytosine-5)-methyltransferase-like protein 2; DNA MTase homolog HsaIIP; DNA methyltransferase-2; tRNA (cytosine-5-)-methyltransferase
UniProt Protein Name
tRNA (cytosine(38)-C(5))-methyltransferase
Protein Family
UniProt Gene Name
TRDMT1
UniProt Synonym Gene Names
DNMT2; Dnmt2; DNA MTase homolog HsaIIP; M.HsaIIP
UniProt Entry Name
TRDMT_HUMAN
NCBI Description
This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]
Uniprot Description
TRDMT1: Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Belongs to the C5-methyltransferase family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.1.1.204; Methyltransferase, tRNA; Amino Acid Metabolism - cysteine and methionine; Methyltransferase
Chromosomal Location of Human Ortholog: 10p15.1
Cellular Component: cytoplasm; nucleus
Molecular Function: DNA (cytosine-5-)-methyltransferase activity; DNA binding; RNA binding; tRNA methyltransferase activity
Biological Process: response to amphetamine; tRNA methylation
Research Articles on TRDMT1
Similar Products
Product Notes
The TRDMT1 trdmt1 (Catalog #AAA8101385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DNMT2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNMT2 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:1500-1:50,000. Researchers should empirically determine the suitability of the TRDMT1 trdmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNMT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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