Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody.)
anti-Mouse, Rat Ras-GRF1 Antibody | anti-Ras-GRF1 antibody
Ras-GRF1 (Phospho-Ser916) Antibody
Gene Names
RASGRF1; GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
Reactivity
Mouse, Rat
Applications
Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
Ras-GRF1; Ras-GRF1 (Phospho-Ser916) Antibody; CDC25; GNRP; GRF1; Guanine nucleotide releasing protein; P140 Ras-GRF; RASGRF1; Ras-specific nucleotide exchange factor CDC25; anti-Ras-GRF1 antibody
Reactivity
Mouse, Rat
Specificity
Ras-GRF1 (Phospho-Ser916) antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1273
Applicable Applications for anti-Ras-GRF1 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
ELISA: 1:20000
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Ras-GRF1 around the phosphorylation site of serine 916 (R-M-SP-L-A).
All Sites
Mouse: Ser916
Rat: Ser898
Rat: Ser898
Preparation and Storage
Store at-20 degree C for 1 year.
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody.)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody.)
Product Categories/Family for anti-Ras-GRF1 antibody
References
Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278-13285. Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177-2189. Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379. Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816-14826.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
143,218 Da
NCBI Official Full Name
ras-specific guanine nucleotide-releasing factor 1 isoform 1
NCBI Official Synonym Full Names
Ras protein specific guanine nucleotide releasing factor 1
NCBI Official Symbol
RASGRF1
NCBI Official Synonym Symbols
GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
NCBI Protein Information
ras-specific guanine nucleotide-releasing factor 1
UniProt Protein Name
Ras-specific guanine nucleotide-releasing factor 1
UniProt Gene Name
RASGRF1
UniProt Synonym Gene Names
CDC25; GNRP; GRF1; Ras-GRF1; GNRP
NCBI Description
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]
Uniprot Description
Promotes the exchange of Ras-bound GDP by GTP.
Research Articles on Ras-GRF1
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Product Notes
The Ras-GRF1 rasgrf1 (Catalog #AAA851601) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Ras-GRF1 (Phospho-Ser916) Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ras-GRF1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-1:100 ELISA: 1:20000. Researchers should empirically determine the suitability of the Ras-GRF1 rasgrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ras-GRF1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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