Mouse anti-Mouse Connexin 47 Monoclonal Antibody | anti-cx47.1 antibody

Connexin 47

Cat. Num. AAA601521

• Gene Names: cx47.1; zgc:92348
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Highly purified from mouse ascites by protein A chromatography.
• Synonyms: Connexin 47; Monoclonal Antibody; Anti -Connexin 47; anti-cx47.1 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4H308
• Specificity: Reacts with the mouse Connexin 47 protein. On Western blots of mouse thalamus homogenates and Connexin 47-transfected HeLa cells, it identified a band at ~47kD. Reactivity was confirmed in Western blots with mouse thalamus lysates. Mouse Connexin 47-transfected HeLa cells were used in both Immunoprecipitation and Western blot assays. This antibody was also used in immunofluorescence assays using paraformaldehyde-fixed mouse brain. The expected staining pattern is punctate labeling of the oligodendrocytes in the brain.
• Purity/Purification: Affinity Purified; Highly purified from mouse ascites by protein A chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-cx47.1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation.; Dilution: Immunofluorescence: 1-3ug/ml; Western Blotting: 1-3ug/ml; Immunoprecipitation: 5-10ug/ml
• Immunogen: Synthetic peptide derived from the C-terminal region of the mouse Connexin 47 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of (A) mouse thalamus homogenates and (B) connexin47 cDNA-transfected HeLa cells using MBS601521)

Related Product Information for anti-cx47.1 antibody

Intercellular communication through gap junctions (connexins) plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation and growth control.1-3 In rodents, 15 connexin genes have been described4,9. Specific cell types in the brain express specific types of connexins; expression patterns coincide with tissue compartmentalization and function. These compartments change during development5. Reduction or alteration in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis10. The coding sequence of the mouse gap junction Cx47 has 49% homology with mouse Cx45; both are located on chromosome 11 in the mouse genome6,8. Cx47 has been observed to express mainly in the gray matter of the central nervous system, specifically in several types of hippocampal, cortical, cerebellar and spinal cord neurons8. Functional gap junctions are involved in tissue homeostasis of ions, metabolites and second messenger molecules10. Between neuronal cells, these functions play a role in synchronizing oscillations of certain cell clusters and could impact

Product Categories/Family for anti-cx47.1 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 52219002
• NCBI GeneID: 447835
• NCBI Accession #: NP_001004574.1
• NCBI GenBank Nucleotide #: NM_001004574.1
• UniProt Accession #: Q66HV7
• Molecular Weight: 47,125 Da
• NCBI Official Full Name: connexin 47.1
• NCBI Official Synonym Full Names: connexin 47.1
• NCBI Official Symbol: cx47.1
• NCBI Official Synonym Symbols: zgc:92348
• NCBI Protein Information: connexin 47.1
• UniProt Protein Name: Gap junction protein
• UniProt Gene Name: cx47.1
• UniProt Entry Name: Q66HV7_DANRE

Uniprot Description

Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell

By similarity. RuleBase RU000630

Subunit structure: A connexon is composed of a hexamer of connexins

By similarity. RuleBase RU000630

Subcellular location: Membrane; Multi-pass membrane protein

By similarity RuleBase RU000630.

Sequence similarities: Belongs to the connexin family. RuleBase RU000630