Flow Cytometry (FC/FACS)
(Staining of SJL splenocytes with 0.25 ug of Mouse IgG2a K Isotype Control (open histogram) or 0.25 ug of CD45.1 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Cells in the lymphocyte gate were used for analysis.)
Mouse anti-Mouse CD45.1 Monoclonal Antibody | anti-CD45.1 antibody
Mouse monoclonal CD45.1 antibody
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Functional Assay, Immunoprecipitation
Purity
Purified by affinity chromatography.
Synonyms
CD45.1; Monoclonal Antibody; Mouse monoclonal CD45.1 antibody; CD45.1 antibody; anti-CD45.1 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
A20
Specificity
Mouse
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-CD45.1 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Functional Assay, Immunoprecipitation (IP)
Application Notes
FC: <= 0.5 ug/test
Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10? to 10? cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of SJL splenocytes with 0.25 ug of Mouse IgG2a K Isotype Control (open histogram) or 0.25 ug of CD45.1 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of SJL splenocytes with 0.25 ug of Mouse IgG2a K Isotype Control (open histogram) or 0.25 ug of CD45.1 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Cells in the lymphocyte gate were used for analysis.)
Product Categories/Family for anti-CD45.1 antibody
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Product Notes
The CD45.1 (Catalog #AAA5305570) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal CD45.1 antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD45.1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Functional Assay, Immunoprecipitation (IP). FC: <= 0.5 ug/test. Researchers should empirically determine the suitability of the CD45.1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45.1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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