Hamster DELTA-LIKE PROTEIN 4 Monoclonal Antibody | anti-Dll4 antibody

HAMSTER ANTI MOUSE DELTA-LIKE PROTEIN 4:RPE

Cat. Num. AAA219928

• Gene Names: Dll4; Delta4
• Applications: Flow Cytometry, Functional Assay
• Synonyms: DELTA-LIKE PROTEIN 4; Monoclonal Antibody; HAMSTER ANTI MOUSE DELTA-LIKE PROTEIN 4:RPE; anti-Dll4 antibody

• Host: Hamster
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: HMD4-2
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Sequence Length: 686

• Applicable Applications for anti-Dll4 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 1.0 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. ; Perservative Stabilisers
• Immunogen: Recombinant mouse DLL4; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution

Related Product Information for anti-Dll4 antibody

Hamster anti Mouse Delta-Like Protein 4 antibody, clone HMD4-2 specifically recognizes mouse Delta-like protein 4 (DLL4), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumours, and pancreatic, bladder and colonic cancer. Studies have shown that DLL4 expression in endothelium cells, can be up-regulated by vascular endothelial growth factor (VEGF) and basic-FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumour growth by promoting non-productive angiogenesis. Hamster anti Mouse Delta-Like Protein 4 ant

NCBI and Uniprot Product Information

• NCBI GI #: 244799228
• NCBI GeneID: 54485
• NCBI Accession #: NP_062327.2
• NCBI GenBank Nucleotide #: NM_019454.3
• UniProt Accession #: Q9JI71; Q9DBU9; Q9JHZ7
• Molecular Weight: 74,991 Da
• NCBI Official Full Name: delta-like protein 4
• NCBI Official Synonym Full Names: delta-like 4 (Drosophila)
• NCBI Official Symbol: Dll4
• NCBI Official Synonym Symbols: Delta4
• NCBI Protein Information: delta-like protein 4; drosophila Delta homolog 4
• UniProt Protein Name: Delta-like protein 4
• Protein Family: Delta-like protein
• UniProt Gene Name: Dll4
• UniProt Synonym Gene Names: Delta4
• UniProt Entry Name: DLL4_MOUSE

Uniprot Description

DLL4: Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4. Binds to Notch-1 and Notch-4. Expressed in vascular endothelium.

Protein type: Cell development/differentiation; Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; integral to membrane

Molecular Function: protein binding; calcium ion binding; Notch binding

Biological Process: nervous system development; Notch signaling pathway; multicellular organismal development; response to stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of blood vessel endothelial cell migration; patterning of blood vessels; negative regulation of cell proliferation; regulation of neurogenesis; visual perception; cell communication; blood vessel remodeling; angiogenesis; cell differentiation