Rat anti-Mouse Dectin 2 Monoclonal Antibody | anti-Clec4n antibody

Dectin 2 (Dendritic Cell-associated C-type Lectin 2, DC-associated C-type Lectin 2, Dectin-2, C-type Lectin Domain Family 6 Member A, CLEC6A, C-type Lectin Superfamily Member 10, CLECSF10) (MaxLight 750)

Cat. Num. AAA6256936

• Gene Names: Clec4n; Nkcl; Clec6a; Clecsf10
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by protein G affinity chromatography from tissue culture supernatant.
• Synonyms: Dectin 2; Monoclonal Antibody; Dectin 2 (Dendritic Cell-associated C-type Lectin 2; DC-associated C-type Lectin 2; Dectin-2; C-type Lectin Domain Family 6 Member A; CLEC6A; C-type Lectin Superfamily Member 10; CLECSF10) (MaxLight 750); Clec4n; anti-Clec4n antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 10B2695
• Specificity: Recognizes mouse Dectin 2. Does not stain brain microglia or meningeal macrophages.
• Purity/Purification: Purified by protein G affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight750.

• Applicable Applications for anti-Clec4n antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Dectin 2 (extracellular domain) Fc Fusion protein.
• Conjugate: MaxLight750
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in mouse spleen stained with D1876-49D.)

Testing Data

(Assessment of dectin-2 function on inflammatory cells. Assessment of the impact of blockade of dectin-2 on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/MØ. Data represents mean±SEM of triplicates from a representative of 3 independent experiments.)

Flow Cytometry (FC/FACS)

(Inhibition of dectin-1 and -2-mediated fungal recognition by prior complement opsonization. A) Representative flow cytometric plots of dectin-2 surface expression on NIH3T3 retrovirally transduced with dectin-2 (left panel) and both dectin-2 and FceR chain (right panel). B) Serum-opsonization inhibits the recognition of zymosan by both dectin-1 and dectin-2. NIH3T3 retrovirally transduced as indicated in the legend were incubated with zymosan that had been pre-incubated with serum at increasing concentrations and the interaction was monitored by flow cytometry. Data represents the mean±SEM of triplicates from a representative of 2 independent experiments. Data in (D) was analyzed by two-way ANOVA, with the significant Interaction statistics demonstrating the reduced binding of zymosan to the transduced cells after prior incubation with increasing concentrations of serum.)

Testing Data

(Assessment of dectin-2 function on inflammatory cells. A) Flow-cytometric plots showing expression of dectin-2 on the surface of inflammatory neutrophils (Ly-6G+) and monocyte/MØ (Ly-6G-). Data is pre-gated on Ly-6B.2+ cells and is representative of results obtained with pooled cells from 3 independent experiments. B) Assessment of the impact of blockade of dectin-2 on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/MØ from dectin-1-deficient mice. All bars show mean±95% confidence intervals of dectin-2-blocked cells relative to isotype control treated)

Product Categories/Family for anti-Clec4n antibody

Antibodies; Lectins

References

1. Taylor, P.R. et al. (2005) Dectin-2 is predominantly myeloid restricted and exhibits unique activation-dependent expression on maturing inflammatory monocytes elicited in vivo. Eur J Immunol. 35:2163 - 2174. 2. Suram, S. et al. (2010) Pathways regulating cytosolic phospholipase A2 activation and eicosanoid production in macrophages by Candida albicans. J Biol Chem. 285: 30676-85. 3. Barrett, N.A. et al. (2011) Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes. J Exp Med. 208: 593-604. 4. Robinson, M. et al. (2009) Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection. J Exp Med. 206:2037-51. 5. McDonald, J.U. et al. (2012) Differential dependencies of monocytes and neutrophils on dectin-1, dectin-2 and complement for the recognition of fungal particles in inflammation. PLoS One. 7: e45781. 6. Ritter, M. et al. (2010) Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses. Proc Natl Acad Sci U S A. 107: 20459-64. 7. Pugholm, L.H. et al. (2015) In vitro Assay for Screening of Optimal Targets for Antigen-Delivery to Murine Dendritic Cells. Scand J Immunol. 82 (6): 498-505. 8. Lee, M.J. et al. (2016) Phosphoinositide 3-Kinase delta Regulates Dectin-2 Signaling and the Generation of Th2 and Th17 Immunity. J Immunol. 197 (1): 278-87. 9. Xiao, Y. et al. (2016) Targeting CBLB as a potential therapeutic approach for disseminated candidiasis. Nat Med. Jul 18. 10. Roth, S. et al. (2016) Vav Proteins Are Key Regulators of Card9 Signaling for Innate Antifungal Immunity Cell Reports. 17 (10): 2572-83. 11. Simons, B. et al. (2017) PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen. J Immunol. 198 (1): 461-71. 12. Chang, T.H. et al. (2017) Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum. PLoS Pathog. 13 (7): e1006485.

NCBI and Uniprot Product Information

• NCBI GI #: 298358452
• NCBI GeneID: 56620
• NCBI Accession #: NP_001177250.1
• NCBI GenBank Nucleotide #: NM_001190321.1
• UniProt Accession #: Q9JKF4; Q9JKF2; Q9JKF3
• Molecular Weight: 19,332 Da
• NCBI Official Full Name: C-type lectin domain family 6 member A isoform 3
• NCBI Official Synonym Full Names: C-type lectin domain family 4, member n
• NCBI Official Symbol: Clec4n
• NCBI Official Synonym Symbols: Nkcl; Clec6a; Clecsf10
• NCBI Protein Information: C-type lectin domain family 6 member A; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 10; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin-2; dendritic cell-associated C-type lectin 2; nat
• UniProt Protein Name: C-type lectin domain family 6 member A
• UniProt Gene Name: Clec6a
• UniProt Synonym Gene Names: Clec4n; Clecsf10; Dectin2; DC-associated C-type lectin 2; Dectin-2
• UniProt Entry Name: CLC6A_MOUSE