Rabbit anti-Human HSPH1 Polyclonal Antibody | anti-HSPH1 antibody

HSPH1, CT (Heat Shock Protein 105kD, Heat Shock 110kD Protein, Antigen NY-CO-25, HSP105, HSP110, KIAA0201) (FITC)

Cat. Num. AAA6479831

• Gene Names: HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HSPH1; Polyclonal Antibody; CT (Heat Shock Protein 105kD; Heat Shock 110kD Protein; Antigen NY-CO-25; HSP105; HSP110; KIAA0201) (FITC); anti-HSPH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HSPH1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HSPH1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human HSPH1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of HSPH1 Antibody (C-term) in A2058 and A375 cell line lysates (35ug/lane). HSPH1 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-HSPH1 antibody

Antibodies; Heat Shock Proteins

References

1. Ishihara K., Yasuda K., Hatayama T.Biochim. Biophys. Acta 1444:138-142(1999). 2. Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., DNA Res. 3:321-329(1996). 3. The MGC Project Team Genome Res. 14:2121-2127(2004). 4. Miyazaki M., Nakatsura T., Yokomine K., Senju S., Monji M., Hosaka S., Cancer Sci. 96:695-705(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 42544159
• NCBI GeneID: 10808
• NCBI Accession #: NP_006635
• NCBI GenBank Nucleotide #: NM_006644.3
• UniProt Accession #: Q92598; O95739; Q5TBM6; Q5TBM7; Q5TBM8; Q9UPC4; B4DYH1
• Molecular Weight: 97,460 Da
• NCBI Official Full Name: heat shock protein 105 kDa isoform 1
• NCBI Official Synonym Full Names: heat shock protein family H (Hsp110) member 1
• NCBI Official Symbol: HSPH1
• NCBI Official Synonym Symbols: HSP105; HSP105A; HSP105B; NY-CO-25
• NCBI Protein Information: heat shock protein 105 kDa
• UniProt Protein Name: Heat shock protein 105 kDa
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPH1
• UniProt Synonym Gene Names: HSP105; HSP110; KIAA0201
• UniProt Entry Name: HS105_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]

Uniprot Description

HSP105: a molecular chaperone of the heat shock protein 70 family. Has ATPase activity. Expressed at especially high levels in mammalian brain and has been shown to suppress apoptosis in neuronal cells and to prevent the aggregation of proteins following heat shock. Known to interact with a variety of proteins including alpha-tubulin and the androgen receptor. Two alternatively spliced isoforms have been described.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; microtubule; cytoplasm; extracellular region; cytosol

Molecular Function: protein binding; ATP binding; alpha-tubulin binding

Biological Process: receptor-mediated endocytosis; positive regulation of NK T cell activation; chaperone cofactor-dependent protein folding; positive regulation of MHC class I biosynthetic process; response to unfolded protein