Mouse anti-Human DC-SIGN Monoclonal Antibody | anti-CD209 antibody

Anti-DC-SIGN Antibody, Mouse Monoclonal

Cat. Num. AAA8101653

• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: DC-SIGN; Monoclonal Antibody; Anti-DC-SIGN Antibody; Mouse Monoclonal; CD209/DC-SIGN Antibody; Mouse MAb; CD209 molecule; Anti-CD209 Antibody; Anti-CDSIGN Antibody; Anti-CLEC4L Antibody; Anti-DC-SIGN1 Antibody; Anti-MGC129965 Antibody; anti-CD209 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 01
• Specificity: Human CD209/DC-SIGN
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD209 antibody: ELISA (EIA) (Cap), Flow Cytometry (FC/FACS/FCM)
• Application Notes: FC/FACS/FCM: 1:25-1:100; ELISA (Cap): 1:250-1:2000; ELISA (Cap): This antibody will detect Human CD209/DC-SIGN in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody when paired with.
• Immunogen: Recombinant Human CD209/DC-SIGN Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD209/DC-SIGN. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CD209 expression on DC. Cells were stained with purified anti-Human CD209, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-CD209 antibody

Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has an important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates the capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.

References

Geijtenbeek TB, et al. (2002) DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 71(6): 921-31.
Masso M. (2003) DC-SIGN points the way to a novel mechanism for HIV-1 transmission. MedGenMed. 5(2): 2.
Zhou T, et al. (2006) DC-SIGN and immunoregulation. Cell Mol Immunol. 3(4): 279-83.