Mouse anti-Human, Mouse DBC1 Monoclonal Antibody | anti-CCAR2 antibody

DBC1 Antibody

Cat. Num. AAA9215583

• Gene Names: CCAR2; DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunoprecipitation
• Synonyms: DBC1; Monoclonal Antibody; DBC1 Antibody; Purified Mouse Monoclonal Antibody (Mab); DBCCR1; Deleted in bladder cancer protein 1; Deleted in bladder cancer protein 1 recursor; FAM5A; Protein FAM5A.; anti-CCAR2 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
• Sequence Length: 923

• Applicable Applications for anti-CCAR2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB ~~ 1:500; ICC ~~ 1:200; IP ~~ 1:500
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of DBC1 in HeLa,A549,Jurkat,C6,3T3 and COS7 cell lysates using DBC1 mouse mAb (1:500 diluted).Predicted band size:130KDa.Observed band size:130KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry of HeLa cells using anti-DBC1 mouse mAb diluted 1:200.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using DBC1 mouse mAb.)

Related Product Information for anti-CCAR2 antibody

Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. As part of a histone H3- specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation.

Product Categories/Family for anti-CCAR2 antibody

Cancer; Signal Transduction

References

Ota T.,et al.Nat. Genet. 36:40-45(2004). Bechtel S.,et al.BMC Genomics 8:399-399(2007). Nusbaum C.,et al.Nature 439:331-335(2006). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Nagase T.,et al.DNA Res. 8:319-327(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 24432106
• NCBI GeneID: 57805
• NCBI Accession #: NP_066997.3
• NCBI GenBank Nucleotide #: NM_021174.5
• UniProt Accession #: Q8N163; Q6P0Q9; Q8N3G7; Q8N8M1; Q8TF34; Q9H9Q9; Q9HD12; Q9NT55; A6NL03; B2RB79; D3DSR6
• Molecular Weight: 102902
• NCBI Official Full Name: cell cycle and apoptosis regulator protein 2
• NCBI Official Synonym Full Names: cell cycle and apoptosis regulator 2
• NCBI Official Symbol: CCAR2
• NCBI Official Synonym Symbols: DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967
• NCBI Protein Information: cell cycle and apoptosis regulator protein 2
• UniProt Protein Name: Cell cycle and apoptosis regulator protein 2
• Protein Family: Cell cycle and apoptosis regulator protein
• UniProt Gene Name: CCAR2
• UniProt Synonym Gene Names: DBC1; KIAA1967; DBC-1; DBC.1
• UniProt Entry Name: CCAR2_HUMAN

NCBI Description

DBC-1: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Component of the DBIRD complex. Interacts with ZNF326/ZIRD; the interaction is direct. Interacts (via N-terminus) with SIRT1. Interacts (via N-terminus) with SUV39H1; this interaction abolishes the interaction with SIRT1. Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Tumor suppressor; RNA-binding; Nuclear receptor co-regulator; Apoptosis

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: nucleoplasm; spliceosome; mitochondrial matrix; cytoplasm; nuclear chromatin; nucleus

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding

Biological Process: Wnt receptor signaling pathway; transcription, DNA-dependent; positive regulation of apoptosis; RNA splicing; rhythmic process; regulation of protein stability; regulation of circadian rhythm; cell cycle; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of catalytic activity; mitochondrial fragmentation during apoptosis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; mRNA processing; regulation of RNA elongation; response to DNA damage stimulus; response to UV

Uniprot Description

DBC-1: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Component of the DBIRD complex. Interacts with ZNF326/ZIRD; the interaction is direct. Interacts (via N-terminus) with SIRT1. Interacts (via N-terminus) with SUV39H1; this interaction abolishes the interaction with SIRT1. Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Mitochondrial; Apoptosis; RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: cytoplasm; mitochondrial matrix; nuclear chromatin; nucleoplasm; nucleus; spliceosome

Molecular Function: enzyme binding; enzyme inhibitor activity; protein binding

Biological Process: cell cycle; mitochondrial fragmentation during apoptosis; mRNA processing; negative regulation of catalytic activity; negative regulation of cell growth; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of transcription, DNA-dependent; positive regulation of apoptosis; regulation of circadian rhythm; regulation of protein stability; regulation of RNA elongation; response to DNA damage stimulus; response to UV; rhythmic process; RNA splicing; transcription, DNA-dependent; Wnt receptor signaling pathway