Mouse DAND5 Monoclonal Antibody | anti-DAND5 antibody

DAND5 antibody

Cat. Num. AAA533877

• Gene Names: DAND5; SP1; CER2; COCO; CRL2; CERL2; DANTE; GREM3; CKTSF1B3
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: DAND5 antibody was purified by affinity chromatography.
• Synonyms: DAND5; Monoclonal Antibody; DAND5 antibody; Monoclonal DAND5; Anti-DAND5; DAN domain family member 5; CER2; CKTSF1B3; GREM3; DANTE; Cerberus like protein 2; Cysteine knot superfamily 1 BMP antagonist 3; DAND 5; DAND-5; anti-DAND5 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2H3
• Purity/Purification: DAND5 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-DAND5 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IHC: 1:150; WB: 1:500-2000
• Biological Significance: DAND5 is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists containcystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry.
• Cross-Reactivity: Human
• Immunogen: DAND5 antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 23-189 of human DAND5 (NP_689867) produced in E Coli as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DAND5 protein (Right) or empty vector (Left) detected with DAND5 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of DAND5 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using DAND5 antibody)

Related Product Information for anti-DAND5 antibody

Mouse monoclonal DAND5 antibody

Product Categories/Family for anti-DAND5 antibody

Differentiation & Development

NCBI and Uniprot Product Information

• NCBI GI #: 22749329
• NCBI GeneID: 199699
• NCBI Accession #: NP_689867
• NCBI GenBank Nucleotide #: NM_152654.2
• UniProt Accession #: Q8N907
• Molecular Weight: 20,180 Da
• NCBI Official Full Name: DAN domain family member 5
• NCBI Official Synonym Full Names: DAN domain family member 5, BMP antagonist
• NCBI Official Symbol: DAND5
• NCBI Official Synonym Symbols: SP1; CER2; COCO; CRL2; CERL2; DANTE; GREM3; CKTSF1B3
• NCBI Protein Information: DAN domain family member 5
• UniProt Protein Name: DAN domain family member 5
• Protein Family: DAN domain family
• UniProt Gene Name: DAND5
• UniProt Synonym Gene Names: CER2; CKTSF1B3; GREM3; SP1; Cerl-2
• UniProt Entry Name: DAND5_HUMAN

NCBI Description

This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry. [provided by RefSeq, Jul 2008]

Uniprot Description

DAND5: Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, tissue growth and differentiation. Belongs to the DAN family.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular region

Molecular Function: morphogen activity

Biological Process: negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of BMP signaling pathway