Mouse anti-Human DAK Monoclonal Antibody | anti-DAK antibody

DAK (Bifunctional ATP-dependent Dihydroxyacetone Kinase/FAD-AMP Lyase (Cyclizing), ATP-dependent Dihydroxyacetone Kinase, DHA Kinase, Glycerone Kinase, FAD-AMP Lyase (Cyclizing), Triokinase, Triose Kinase, FAD-AMP Lyase (Cyclic FMN Forming), FMN Cyclase)

Cat. Num. AAA6152066

• Gene Names: TKFC; DAK; NET45
• Reactivity: Human
• Applications: ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DAK; Monoclonal Antibody; DAK (Bifunctional ATP-dependent Dihydroxyacetone Kinase/FAD-AMP Lyase (Cyclizing); ATP-dependent Dihydroxyacetone Kinase; DHA Kinase; Glycerone Kinase; FAD-AMP Lyase (Cyclizing); Triokinase; Triose Kinase; FAD-AMP Lyase (Cyclic FMN Forming); FMN Cyclase); anti-DAK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1D9
• Specificity: Recognizes human DAK.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-DAK antibody: ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length recombinant corresponding to aa1-576 from human DAK (AAH01341) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTSKKLVNSVAGCADDALAGLVACNPNLQLLQGHRVALRSDLDSLKGRVALLSGGGSGHEPAHAGFIGKGMLTGVIAGAVFTSPAVGSILAAIRAVAQAGTVGTLLIVKNYTGDRLNFGLAREQARAEGIPVEMVVIGDDSAFTVLKKAGRRGLCGTVLIHKVAGALAEAGVGLEEIAKQVNVVAKAMGTLGVSLSSCSVPGSKPTFELSADEVELGLGIHGEAGVRRIKMATADEIVKLMLDHMTNTTNASHVP
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged DAK is 0.3ng/ml as a capture antibody.)

Related Product Information for anti-DAK antibody

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.

Product Categories/Family for anti-DAK antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 12654986
• NCBI GeneID: 26007
• Molecular Weight: 54,793 Da
• NCBI Official Full Name: Homo sapiens dihydroxyacetone kinase 2 homolog (S. cerevisiae), mRNA
• NCBI Official Synonym Full Names: triokinase and FMN cyclase
• NCBI Official Symbol: TKFC
• NCBI Official Synonym Symbols: DAK; NET45
• NCBI Protein Information: triokinase/FMN cyclase
• Protein Family: Dihydroxyacetone kinase

NCBI Description

This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]