Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using MARCH5 Antibody. The picture on the right is blocked with the synthesized peptide.)
Rabbit MARCH5 Polyclonal Antibody | anti-MARCH5 antibody
MARCH5 Antibody
Gene Names
MARCH5; MITOL; RNF153; MARCH-V
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
MARCH5; Polyclonal Antibody; MARCH5 Antibody; E3 ubiquitin-protein ligase MARCH5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; MARCH-V; RING finger protein 153; anti-MARCH5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
MARCH5 Antibody detects endogenous levels of total MARCH5 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
278
Applicable Applications for anti-MARCH5 antibody
Immunofluorescence (IF), ELISA (EIA)
Application Notes
IF: 1:100~1:500
ELISA: 1:40000
ELISA: 1:40000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human MARCH5.
Immunogen Range
21-70
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using MARCH5 Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using MARCH5 Antibody. The picture on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH5
NCBI Official Synonym Full Names
membrane associated ring-CH-type finger 5
NCBI Official Symbol
MARCH5
NCBI Official Synonym Symbols
MITOL; RNF153; MARCH-V
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH5
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH5
UniProt Gene Name
MARCH5
UniProt Synonym Gene Names
RNF153; MARCH-V; MITOL
NCBI Description
MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008]
Uniprot Description
Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Research Articles on MARCH5
Similar Products
Product Notes
The MARCH5 march5 (Catalog #AAA9506320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARCH5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MARCH5 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA). IF: 1:100~1:500 ELISA: 1:40000. Researchers should empirically determine the suitability of the MARCH5 march5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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