Mouse anti-Human TFIIB Monoclonal Antibody | anti-TFIIB antibody

TFIIB Monoclonal Antibody

Cat. Num. AAA3014246

• Gene Names: GTF2B; TF2B; TFIIB
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TFIIB; Monoclonal Antibody; TFIIB Monoclonal Antibody; TF2B; S300-II; Transcription initiation factor IIB; anti-TFIIB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Specificity: This antibody detects endogenous levels of TFIIB and does not cross-react with related proteins
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 316

• Applicable Applications for anti-TFIIB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; ICC: 1:50-200; IHC: 1:500
• Immunogen: Recombinant full length Human TFIIB.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot detection of TFIIB in Jurkat, MCF7&Hela cell lysates using TFIIB antibody (1:1000 diluted).)

Immunohistochemistry (IHC)

(IHC of paraffin-embedded human colon using anti- TFIIB diluted 1/500-1/1000.)

Immunocytochemistry (ICC)

(Immunocytochemistry of HeLa cells using anti- TFIIB antibody diluted 1:50.)

Related Product Information for anti-TFIIB antibody

Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the "TATA" element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.

NCBI and Uniprot Product Information

• NCBI GI #: 18088837
• NCBI GeneID: 2959
• UniProt Accession #: Q00403
• Molecular Weight: Predicted Band Size: 35KDa; Observed Band Size: 37KDa
• NCBI Official Full Name: General transcription factor IIB
• NCBI Official Synonym Full Names: general transcription factor IIB
• NCBI Official Symbol: GTF2B
• NCBI Official Synonym Symbols: TF2B; TFIIB
• NCBI Protein Information: transcription initiation factor IIB
• UniProt Protein Name: Transcription initiation factor IIB
• UniProt Gene Name: GTF2B
• UniProt Synonym Gene Names: TF2B; TFIIB
• UniProt Entry Name: TF2B_HUMAN

NCBI Description

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]

Uniprot Description

GTF2B: General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. Associates with TFIID-IIA (DA complex) to form TFIID-IIA- IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1. Interacts with Epstein-Barr virus EBNA2. Belongs to the TFIIB family.

Protein type: Transcription factor; Nuclear receptor co-regulator; Acetyltransferase

Chromosomal Location of Human Ortholog: 1p22-p21

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; zinc ion binding; TATA-binding protein binding; thyroid hormone receptor binding; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase II promoter; gene expression