Western Blot (WB)
(Western Blotting Analysis: |Representative lot data. |Jurkat cell lysate was probed with 0.5 µg/mL of Anti-Cytohesin-1, clone 7H2. |Proteins were visualized using a Goat Anti-Rat IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. |Arrow indicates Cytohesin-1 (~46 kD).)
Rat anti-Human Cytohesin 1 Monoclonal Antibody
Cytohesin 1 (B2-1, CLM1, CTH-1, CYTIP, Cytoadhesin 1, D17S811E, Homolog of Secretory Protein SEC7, Pleckstrin Homology Sec7 and Coiled/Coil Domains 1, PSCD1)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: Western Blot: 0.5ug/ml
Western Blot (WB)
(Western Blotting Analysis: |Representative lot data. |Jurkat cell lysate was probed with 0.5 µg/mL of Anti-Cytohesin-1, clone 7H2. |Proteins were visualized using a Goat Anti-Rat IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. |Arrow indicates Cytohesin-1 (~46 kD).)
Western Blot (WB)
(Western Blotting Analysis: |Representative lot data. |Jurkat cell lysate was probed with 0.5 µg/mL of Anti-Cytohesin-1, clone 7H2. |Proteins were visualized using a Goat Anti-Rat IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. |Arrow indicates Cytohesin-1 (~46 kD).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. A pseudogene of this gene has been defined on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Uniprot Description
cytohesin 1: Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: GEFs; GEFs, ARF; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q25
Cellular Component: extrinsic to internal side of plasma membrane; tight junction; cytoplasm; plasma membrane; trans-Golgi network; cytosol
Molecular Function: protein binding; lipid binding; ARF guanyl-nucleotide exchange factor activity
Biological Process: vesicle-mediated transport; regulation of cell adhesion; regulation of ARF protein signal transduction; positive regulation of GTPase activity
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Product Notes
The Cytohesin 1 (Catalog #AAA632369) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Cytohesin 1 (B2-1, CLM1, CTH-1, CYTIP, Cytoadhesin 1, D17S811E, Homolog of Secretory Protein SEC7, Pleckstrin Homology Sec7 and Coiled/Coil Domains 1, PSCD1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytohesin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Dilution: Western Blot: 0.5ug/ml. Researchers should empirically determine the suitability of the Cytohesin 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytohesin 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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