Human Growth Factor Receptor Bound Protein 2 (Grb2) ELISA Kit | Grb2 elisa kit

Human Growth Factor Receptor Bound Protein 2 (Grb2) ELISA Kit

Cat. Num. AAA2022056

• Reactivity: Human
• Synonyms: Growth Factor Receptor Bound Protein 2 (Grb2); Human Growth Factor Receptor Bound Protein 2 (Grb2) ELISA Kit; ASH; NCKAP2; EGFRBP-GRB2; Grb3-3; Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2; Grb2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Factor Receptor Bound Protein 2 (Grb2).; No significant cross-reactivity or interference between Growth Factor Receptor Bound Protein 2 (Grb2) and analogues was observed.
• Sequence Length: 59

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 2 (Grb2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 2 (Grb2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Grb2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Factor Receptor Bound Protein 2 (Grb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Factor Receptor Bound Protein 2 (Grb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Factor Receptor Bound Protein 2 (Grb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Factor Receptor Bound Protein 2 (Grb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for Grb2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 77539774
• NCBI GeneID: 81504
• NCBI Accession #: NP_110473.2
• NCBI GenBank Nucleotide #: NM_030846.2
• UniProt Accession #: P62994; P29354; Q14450; Q5BKA7; Q63057; Q63059
• Molecular Weight: 6,880 Da
• NCBI Official Full Name: growth factor receptor-bound protein 2
• NCBI Official Synonym Full Names: growth factor receptor bound protein 2
• NCBI Official Symbol: Grb2
• NCBI Protein Information: growth factor receptor-bound protein 2
• UniProt Protein Name: Growth factor receptor-bound protein 2
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: Grb2
• UniProt Synonym Gene Names: Ash
• UniProt Entry Name: GRB2_RAT

NCBI Description

acts as a link between tyrosine kinase receptors and Ras signaling [RGD, Feb 2006]

Uniprot Description

GRB2: an adaptor protein of the GRB2 / sem-5 / DRK family. Associates with many different tyrosine-phosphorylated proteins via its SH2 domain, including EGFR, PDGFR, IRS-1, SHC and LNK. It also seems to interact with RAS in the signaling pathway leading to DNA synthesis. Binds to and translocates the guanine nucleotide exchange factors SOS. Four alternatively spliced isoforms have been described. Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Protein type: Cell development/differentiation; Adaptor/scaffold; Motility/polarity/chemotaxis

Cellular Component: signalosome; nucleoplasm; Golgi apparatus; protein complex; membrane; cytoplasm; nucleolus; plasma membrane; vesicle membrane; intercellular junction; nucleus; cytosol; endosome

Molecular Function: protein domain specific binding; identical protein binding; protein binding; SH3/SH2 adaptor activity; ephrin receptor binding; phosphotyrosine binding; insulin receptor substrate binding; neurotrophin TRKA receptor binding; phosphoprotein binding; SH3 domain binding; protein kinase binding; protein phosphatase binding; epidermal growth factor receptor binding

Biological Process: fibroblast growth factor receptor signaling pathway; positive regulation of signal transduction; positive regulation of actin filament polymerization; protein heterooligomerization; regulation of MAPKKK cascade; receptor internalization; Ras protein signal transduction; DNA damage response, signal transduction; insulin receptor signaling pathway; anatomical structure formation; cell differentiation; aging