Rabbit Cytochrome C Monoclonal Antibody | anti-CYC antibody

Cytochrome C Antibody

Cat. Num. AAA9435727

• Gene Names: CYCS; CYC; HCS; THC4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Cytochrome C; Monoclonal Antibody; Cytochrome C Antibody; CYC antibody; CYC_HUMAN antibody; CYCS antibody; Cytochrome c antibody; Cytochrome c somatic antibody; HCS antibody; THC4 antibody; anti-CYC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: SC59-01
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-CYC antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000-5000; IHC: 1:50-1:200; ICC: 1:50-1:200
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Cytochrome C on different lysates using anti-Cytochrome C antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse kidney Lane 2: Rat kidney)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cytochrome C antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Cytochrome C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-CYC antibody

Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Product Categories/Family for anti-CYC antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 11128019
• NCBI GeneID: 54205
• NCBI Accession #: NP_061820.1
• NCBI GenBank Nucleotide #: NM_018947.5
• UniProt Accession #: P99999; P00001; Q6NUR2; Q6NX69; Q96BV4; A4D166; B2R4I1
• Molecular Weight: 11,749 Da
• NCBI Official Full Name: cytochrome c
• NCBI Official Synonym Full Names: cytochrome c, somatic
• NCBI Official Symbol: CYCS
• NCBI Official Synonym Symbols: CYC; HCS; THC4
• NCBI Protein Information: cytochrome c
• UniProt Protein Name: Cytochrome c
• Protein Family: Cytochrome
• UniProt Gene Name: CYCS
• UniProt Synonym Gene Names: CYC
• UniProt Entry Name: CYC_HUMAN

NCBI Description

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Uniprot Description

CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Oxidoreductase; Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: protein phosphatase type 2A complex; mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; protein binding; iron ion binding; heme binding; protein serine/threonine phosphatase activity

Biological Process: mitochondrion organization and biogenesis; cellular metabolic process; response to reactive oxygen species; dephosphorylation; apoptosis; organelle organization and biogenesis; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; DNA fragmentation during apoptosis; caspase activation via cytochrome c; transmembrane transport; mitochondrial electron transport, ubiquinol to cytochrome c

Disease: Thrombocytopenia 4