Typical Testing Data/Standard Curve (for reference only)
Sheep Claudin 1 ELISA Kit | CLDN1 elisa kit
Sheep Claudin 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CLDN-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLDN-1 antibody and an CLDN-1-HRP conjugate. The assay sample and buffer are incubated together with CLDN-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN-1 concentration since CLDN-1 from samples and CLDN-1-HRP conjugate compete for the anti-CLDN-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN-1 from the sample, fewer sites are left to bind CLDN-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN-1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
Claudin-1: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Acts as a co- receptor for HCV entry into hepatic cells. Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL. May interact with HCV E1 and E2 proteins. Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. Belongs to the claudin family.
Protein type: Cytoskeletal; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q28-q29
Cellular Component: tight junction; integral to plasma membrane; apical plasma membrane; integral to membrane; lateral plasma membrane
Molecular Function: identical protein binding; protein binding; structural molecule activity
Biological Process: intercellular junction assembly and maintenance; viral reproduction; cell adhesion; calcium-independent cell-cell adhesion
Disease: Ichthyosis, Leukocyte Vacuoles, Alopecia, And Sclerosing Cholangitis
Research Articles on CLDN1
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Product Notes
The Sheep CLDN1 cldn1 (Catalog #AAA737793) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA737793 ELISA Kit recognizes Sheep CLDN1. It is sometimes possible for the material contained within the vial of "Claudin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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