Western Blot (WB)
Mouse anti-Human Cytochrome b245 Light Chain Monoclonal Antibody
Mouse Anti-Cytochrome b245 Light Chain [CS9]
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Protein G purified
Synonyms
Cytochrome b245 Light Chain; Monoclonal Antibody; Mouse Anti-Cytochrome b245 Light Chain [CS9]; Superoxide-generating NADPH oxidase light chain subunit antibody; CYBA antibody; Cytochrome b(558) alphachain antibody; Cytochrome b558 subunit alpha antibody; Neutrophil cytochrome b 22 kDa polypeptide antibody; p22phagocyte B-cytochrome antibody; p22-phox antibody; p22phox antibody; anti-Cytochrome b245 Light Chain antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CS9
Specificity
p22phox 165-KKPSE-169
Purity/Purification
Protein G purified
Form/Format
Affinity purified antiserum containing PBS 0.1% sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Cytochrome b245 Light Chain antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS), Indirect ELISA (EIA)
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try; WB: Use at 1:1000. Predicted molecular weight: 21 kDa
Target Antigen
Cytochrome b245 Light Chain
Immunogen
Partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in octyl glucoside)
Myeloma/fusion Partners
P3U1 myeloma
Western Blot (WB)
Western Blot (WB)
References
Taylor, R.M. Jesaitis, A.J. Methods Mol Biol. 2007;412:429-37, 2007.Immunoaffinity purification of human phagocyte flavocytochrome b and analysis of conformational dynamics.PMID: 18453126 Taylor RM, et al. J Immunol. 2004 Dec 15;173(12):7349-57. Site-specific inhibitors of NADPH oxidase activity and structural probes of flavocytochrome b: characterization of six monoclonal antibodies to the p22phox subunit.PMID: 15585859
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Product Notes
The Cytochrome b245 Light Chain (Catalog #AAA375213) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Cytochrome b245 Light Chain [CS9] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome b245 Light Chain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS), Indirect ELISA (EIA). Optimal antibody dilution should be determined by titration, however as a guideline try; WB: Use at 1:1000. Predicted molecular weight: 21 kDa. Researchers should empirically determine the suitability of the Cytochrome b245 Light Chain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome b245 Light Chain, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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