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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Mouse anti-Human Cystatin 2 Monoclonal Antibody | anti-CST2 antibody

Monoclonal Antibody to Cystatin 2 (CST2)

Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
Cystatin 2; Monoclonal Antibody; Monoclonal Antibody to Cystatin 2 (CST2); anti-CST2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C1
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CST2 antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2 ug/mL;1:500-2000
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200

Optimal working dilutions must be determined by end user.
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant CST2 (Trp21~Ala141 (Accession # P09228)) expressed in E.coli
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2081181
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cystatin-SA
NCBI Official Synonym Full Names
cystatin SA
NCBI Official Symbol
CST2
NCBI Protein Information
cystatin-SA
UniProt Protein Name
Cystatin-SA
Protein Family
UniProt Gene Name
CST2

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a secreted thiol protease inhibitor found at high levels in saliva, tears and seminal plasma. [provided by RefSeq, Jul 2008]

Uniprot Description

CST2: Thiol protease inhibitor. Belongs to the cystatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space

Molecular Function: cysteine-type endopeptidase inhibitor activity; protease binding; protein binding

Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste

Research Articles on CST2

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Product Notes

The CST2 cst2 (Catalog #AAA2025776) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Cystatin 2 (CST2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cystatin 2 can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2 ug/mL;1:500-2000 Immunohistochemistry: 5-20 ug/mL;1:50-200 Immunocytochemistry: 5-20 ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CST2 cst2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cystatin 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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