Western Blot (WB)
(Staining (0.5ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.)
Goat ATF2 Polyclonal Antibody | anti-ATF2 antibody
Goat anti-ATF2 Antibody
Western Blot: Approx 55kDa and 30kDa bands observed in lysates of Human Placenta. These bands correspond to earlier findings in literature (Bailey et al, J Clin Endocrinol Metab.2002 Apr;87(4): 1717-28.; PMID: 11932306). Approx 75kDa observed in nuclear lysates of cell lines HeLa, HepG2 and K562 (calculated MW of 54.5kDa according to NP_001871.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Knippen et al, Anticancer Res.2009 Jan;29(1): 183-189 ; PMID: 19331149). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
(Staining (0.5ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.5ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]
Uniprot Description
ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro.
Protein type: C2H2-type zinc finger protein; Transcription factor; EC 2.3.1.48
Chromosomal Location of Human Ortholog: 2q32
Cellular Component: cytoplasm; mitochondrial outer membrane; nucleoplasm; nucleus
Molecular Function: cAMP response element binding protein binding; chromatin binding; histone acetyltransferase activity; metal ion binding; protein binding; protein heterodimerization activity; protein kinase binding; RNA polymerase II transcription factor activity, enhancer binding; transcription coactivator activity; transcription factor activity
Biological Process: fat cell differentiation; histone acetylation; intra-S DNA damage checkpoint; negative regulation of epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of neuron apoptosis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transforming growth factor-beta2 production; regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to osmotic stress; response to water deprivation; transcription from RNA polymerase II promoter
Research Articles on ATF2
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Product Notes
The ATF2 atf2 (Catalog #AAA420512) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ATF2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ATF2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 55kDa and 30kDa bands observed in lysates of Human Placenta. These bands correspond to earlier findings in literature (Bailey et al, J Clin Endocrinol Metab.2002 Apr;87(4): 1717-28.; PMID: 11932306). Approx 75kDa observed in nuclear lysates of cell lines HeLa, HepG2 and K562 (calculated MW of 54.5kDa according to NP_001871.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Knippen et al, Anticancer Res.2009 Jan;29(1): 183-189 ; PMID: 19331149). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the ATF2 atf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: IVMAPSSQSQ PSGS. It is sometimes possible for the material contained within the vial of "ATF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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