Western Blot (WB)
(Detection of Mouse Cyr61 by Western Blot. Western blot shows lysates of mouse brain tissue (negative control) and mouse heart tissue. PVDF membrane was probed with 2 ?g/mL of Cyr61/CCN1 Antibody followed by HRP-conjugated Anti-Rat IgG Secondary Antibody Specific bands were detected for Cyr61 (at approximately 50 kDa) and cleaved Cyr61 (at approximately 25 kDa).)
Rat anti-Mouse Cyr61/CCN1 Monoclonal Antibody | anti-Cyr61/CCN1 antibody
Cyr61/CCN1 Antibody (Clone 466305)
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Protein A or G purified from hybridoma culture supernatant
Synonyms
Cyr61/CCN1; Monoclonal Antibody; Cyr61/CCN1 Antibody (Clone 466305); CCN1; G1G1; IGFBP-10; anti-Cyr61/CCN1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2B
Purity/Purification
Protein A or G purified from hybridoma culture supernatant
Form/Format
Lyophilized powder. Lyophilized from a 0.2 µm filtered solution in PBS with trehalose.
Applicable Applications for anti-Cyr61/CCN1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 2 ug/ml, IHC: 8-25 ug/ml.
Immunogen
E. Coli derived recombinant mouse Cyr61/CCN1. Asp176-Gly281
Positive Control
Western blot: mouse heart tissue, IHC: mouse trigeminal ganglia.
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Detection of Mouse Cyr61 by Western Blot. Western blot shows lysates of mouse brain tissue (negative control) and mouse heart tissue. PVDF membrane was probed with 2 ?g/mL of Cyr61/CCN1 Antibody followed by HRP-conjugated Anti-Rat IgG Secondary Antibody Specific bands were detected for Cyr61 (at approximately 50 kDa) and cleaved Cyr61 (at approximately 25 kDa).)
Western Blot (WB)
(Detection of Mouse Cyr61 by Western Blot. Western blot shows lysates of mouse brain tissue (negative control) and mouse heart tissue. PVDF membrane was probed with 2 ?g/mL of Cyr61/CCN1 Antibody followed by HRP-conjugated Anti-Rat IgG Secondary Antibody Specific bands were detected for Cyr61 (at approximately 50 kDa) and cleaved Cyr61 (at approximately 25 kDa).)
Immunohistochemistry (IHC)
(Cyr61/CCN1 in Mouse Trigeminal Ganglia. Cyr61/CCN1 was detected in perfusion fixed frozen sections of mouse trigeminal ganglia using 25 ?g/mL Cyr61/CCN1 Antibody O/N at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue).)
Immunohistochemistry (IHC)
(Cyr61/CCN1 in Mouse Trigeminal Ganglia. Cyr61/CCN1 was detected in perfusion fixed frozen sections of mouse trigeminal ganglia using 25 ?g/mL Cyr61/CCN1 Antibody O/N at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue).)
Related Product Information for anti-Cyr61/CCN1 antibody
Monoclonal Antibody to detect Cyr61/CCN1 in mouse samples. Cyr61 is a member of angiogenic and vasculogenic regulators designated CCN proteins, which includes CTGF, Cyr61 homolog, Fisp2. Cyr61 functions as an angiogenic inducer, stimulating cell adhesion and migration and promotes DNA synthesis of human vascular endothelial cells. Elevated expression of Cyr61 is observed during vessel growth, wound healing and chondrocyte differentiation.
Similar Products
Product Notes
The Cyr61/CCN1 (Catalog #AAA841531) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Cyr61/CCN1 Antibody (Clone 466305) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cyr61/CCN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 2 ug/ml, IHC: 8-25 ug/ml. Researchers should empirically determine the suitability of the Cyr61/CCN1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyr61/CCN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
