Rat anti-Mouse Cysteine-rich, Angiogenic Inducer 61 Monoclonal Antibody | anti-CYR61 antibody

Cysteine-rich, Angiogenic Inducer 61 (CYR61)

Cat. Num. AAA602103

• Gene Names: CYR61; CCN1; GIG1; IGFBP10
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Cysteine-rich; Angiogenic Inducer 61; Monoclonal Antibody; Angiogenic Inducer 61 (CYR61); Anti -Cysteine-rich; anti-CYR61 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8H206
• Specificity: Recognizes mouse Cyr61. No crossreactivity with human Cyr1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-CYR61 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot.; Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant mouse Cyr61 is ~5ng/well.; Western Blot: 2ug/ml; Immunohistochemistry: 25ug/ml detects Cyr61 in frozen mouse trigeminal ganglia tissue sections.
• Immunogen: Recombinant corresponding to aa176-281 of mouse Cyr61 expressed in E. coli (P18406).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Detection of mouse Cyr61 with MBS602103 Lysates were prepared from NS0 cells and mouse heart extract in non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. Use of this antibody under reducing conditions is not recommended. The blot was developed with 2ug/ml MBS602103 and chemiluminescent detection substrate.)

Immunohistochemistry (IHC)

(Immunohistochemistry-Cyr61/CCN1 in Mouse Trigeminal Ganglia.)

Related Product Information for anti-CYR61 antibody

Cyr61, also known as IGFBP-10 and CCN1, is a 50kD secreted matrix- and cell-associated glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins aVB3, aVB5, aMB2, and a6B1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176-281, mouse Cyr61 shares 87% and 97% aa sequence identity with human and rat Cyr61, respectively.

Product Categories/Family for anti-CYR61 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 119593604
• NCBI GeneID: 3491
• UniProt Accession #: O00622; O14934; O43775; Q9BZL7; O95694; Q53FA4; Q6FI18; Q9UID7
• Molecular Weight: 42,027 Da
• NCBI Official Full Name: cysteine-rich, angiogenic inducer, 61, isoform CRA_a
• NCBI Official Synonym Full Names: cysteine-rich, angiogenic inducer, 61
• NCBI Official Symbol: CYR61
• NCBI Official Synonym Symbols: CCN1; GIG1; IGFBP10
• NCBI Protein Information: protein CYR61; IBP-10; IGFBP-10; OTTHUMP00000012303; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10
• UniProt Protein Name: Protein CYR61
• Protein Family: Protein
• UniProt Gene Name: CYR61
• UniProt Synonym Gene Names: CCN1; GIG1; IGFBP10
• UniProt Entry Name: CYR61_HUMAN

NCBI Description

CYR61 is a secreted, cysteine-rich, heparin-binding protein encoded by a growth factor-inducible immediate-early gene. Acting as an extracellular, matrix-associated signaling molecule, CYR61 promotes the adhesion of endothelial cells through interaction with integrin and augments growth factor-induced DNA synthesis in the same cell type.[supplied by OMIM]

Uniprot Description

CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; extracellular matrix binding

Biological Process: extracellular matrix organization and biogenesis; anatomical structure morphogenesis; positive regulation of caspase activity; chemotaxis; intussusceptive angiogenesis; osteoblast differentiation; cell proliferation; positive regulation of osteoblast differentiation; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell growth; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of BMP signaling pathway; negative regulation of apoptosis; positive regulation of cell migration