Mouse anti-Human CSRP3 Monoclonal Antibody | anti-CSRP3 antibody

CSRP3 (Cysteine and Glycine-rich Protein 3, Cardiac LIM Protein, Cysteine-rich Protein 3, CRP3, LIM Domain Protein, Cardiac, Muscle LIM Protein, CLP, MLP) (PE)

Cat. Num. AAA6157295

• Gene Names: CSRP3; CLP; MLP; CRP3; LMO4; CMD1M; CMH12
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CSRP3; Monoclonal Antibody; CSRP3 (Cysteine and Glycine-rich Protein 3; Cardiac LIM Protein; Cysteine-rich Protein 3; CRP3; LIM Domain Protein; Cardiac; Muscle LIM Protein; CLP; MLP) (PE); anti-CSRP3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 6D2
• Specificity: Recognizes human CSRP3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CSRP3 antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa95-195 from CSRP3 (NP_003467) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: SPKPARSVTTSNPSKFTAKFGESEKCPRCGKSVYAAEKVMGGGKPWHKTCFRCAICGKSLESTNVTDKDGELYCKVCYAKNFGPTGIGFGGLTQQVEKKE
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.11kD).)

Western Blot (WB)

(Western Blot analysis of CSRP3 expression in transfected 293T cell line by CSRP3 monoclonal antibody Lane 1: CSRP3 transfected lysate (20.969kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CSRP3 on HeLa cell. [antibody concentration 10ug/ml])

Western Blot (WB)

(Western blot analysis of CSRP3 over-expressed 293 cell line, cotransfected with CSRP3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSRP3 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Product Categories/Family for anti-CSRP3 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4502893
• NCBI GeneID: 8048
• NCBI Accession #: NP_003467
• NCBI GenBank Nucleotide #: NM_003476
• UniProt Accession #: P50461
• Molecular Weight: 21kDa
• NCBI Official Full Name: cysteine and glycine-rich protein 3
• NCBI Official Synonym Full Names: cysteine and glycine rich protein 3
• NCBI Official Symbol: CSRP3
• NCBI Official Synonym Symbols: CLP; MLP; CRP3; LMO4; CMD1M; CMH12
• NCBI Protein Information: cysteine and glycine-rich protein 3
• UniProt Protein Name: Cysteine and glycine-rich protein 3
• Protein Family: Cysteine and glycine-rich protein
• UniProt Gene Name: CSRP3
• UniProt Synonym Gene Names: CLP; MLP; CRP3
• UniProt Entry Name: CSRP3_HUMAN

NCBI Description

This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CSRP3: Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation. Defects in CSRP3 are the cause of cardiomyopathy dilated type 1M (CMD1M). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in CSRP3 are the cause of familial hypertrophic cardiomyopathy type 12 (CMH12). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoskeleton; nucleus; Z disc

Molecular Function: actinin binding; protein binding; telethonin binding; zinc ion binding; structural constituent of muscle

Biological Process: cardiac muscle development; cellular calcium ion homeostasis; skeletal muscle development; protein localization in organelle; cardiac myofibril assembly; positive regulation of transcription from RNA polymerase II promoter; regulation of the force of heart contraction; cardiac muscle contraction

Disease: Cardiomyopathy, Familial Hypertrophic, 12; Cardiomyopathy, Dilated, 1m