Western Blot (WB)
(Western blot analysis of extracts from Ramos, NTERA2, COS7, Jurkat and Hela cell lysates using Csk mouse mAb (1:1000 diluted). Predicted band size:50KDa. Observed band size:50KDa.)
Mouse Csk Monoclonal Antibody | anti-Csk antibody
Anti-Csk Mouse mAb
Western Blot (WB)
(Western blot analysis of extracts from Ramos, NTERA2, COS7, Jurkat and Hela cell lysates using Csk mouse mAb (1:1000 diluted). Predicted band size:50KDa. Observed band size:50KDa.)
Western Blot (WB)
(Western blot analysis of extracts from Ramos, NTERA2, COS7, Jurkat and Hela cell lysates using Csk mouse mAb (1:1000 diluted). Predicted band size:50KDa. Observed band size:50KDa.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue)
Immunofluorescence (IF)
(Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).)
UniPort Summary Function: Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
NCBI and Uniprot Product Information
Uniprot Description
CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.
Protein type: Csk family; EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group
Chromosomal Location of Human Ortholog: 15q24.1
Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; plasma membrane
Molecular Function: identical protein binding; kinase activity; non-membrane spanning protein tyrosine kinase activity; protein binding; protein C-terminus binding; protein-tyrosine kinase activity; receptor binding
Biological Process: cell differentiation; cell migration; central nervous system development; epidermal growth factor receptor signaling pathway; innate immune response; negative regulation of Golgi to plasma membrane protein transport; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on Csk
Similar Products
Product Notes
The Csk csk (Catalog #AAA476083) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Csk Mouse mAb reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Csk can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Csk csk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Csk, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
