Rabbit CSK Polyclonal Antibody | anti-CSK antibody

CSK Antibody

Cat. Num. AAA9408883

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CSK; Polyclonal Antibody; CSK Antibody; Tyrosine-protein kinase CSK; EC 2.7.10.2; C-SRC kinase; Protein-tyrosine kinase CYL; anti-CSK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total CSK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 450

• Applicable Applications for anti-CSK antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:3000; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human CSK.
• Target Name: CSK
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using CSK antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using CSK antibody.)

Related Product Information for anti-CSK antibody

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.Partanen J., Oncogene 6:2013-2018(1991).Braeuninger A., Proc. Natl. Acad. Sci. U.S.A. 88:10411-10415(1991).Brauninger A., Gene 110:205-211(1992).

Product Categories/Family for anti-CSK antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 187475373
• NCBI GeneID: 1445
• NCBI Accession #: NP_001120662.1
• NCBI GenBank Nucleotide #: NM_001127190.1
• UniProt Accession #: P41240; Q2M3N2; Q6FGZ6
• Molecular Weight: 50,704 Da
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: c-src tyrosine kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK
• UniProt Entry Name: CSK_HUMAN

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; Protein kinase, TK; TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of phagocytosis; platelet activation; negative regulation of kinase activity; cell migration; central nervous system development; negative regulation of Golgi to plasma membrane protein transport; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; positive regulation of MAP kinase activity; negative regulation of cell proliferation; T cell costimulation; negative regulation of interleukin-6 production; oligodendrocyte differentiation; morphogenesis of an epithelium; innate immune response; brain development; blood coagulation; cell differentiation; negative regulation of bone resorption; transmembrane receptor protein tyrosine kinase signaling pathway