Western Blot (WB)
(The extracts of mouse eye (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MCF-7 cell lysate The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: CRABP1 transfected 293T cell lysate)
Mouse anti-Human, Mouse CRABP1 Monoclonal Antibody | anti-CRABP1 antibody
CRABP1 antibody
Recommended starting dilution is 1:500
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The extracts of mouse eye (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MCF-7 cell lysate The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: CRABP1 transfected 293T cell lysate)
Western Blot (WB)
(The extracts of mouse eye (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MCF-7 cell lysate The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: CRABP1 transfected 293T cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of CRABP1 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CRABP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of CRABP1 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CRABP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a specific binding protein for a vitamin A family member and is thought to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid at specific sites within the nucleus, which may contribute to vitamin A-directed differentiation in epithelial tissue. [provided by RefSeq, Jul 2008]
Uniprot Description
Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.
Research Articles on CRABP1
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Product Notes
The CRABP1 crabp1 (Catalog #AAA200159) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CRABP1 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CRABP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500 ~ 1000. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the CRABP1 crabp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRABP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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