Human Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit | HADHb elisa kit

Human Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit

Cat. Num. AAA457042

• Gene Names: HADHB; ECHB; MTPB; MSTP029; TP-BETA
• Reactivity: Human
• Synonyms: Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb); Human Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit; MTPB; Mitochondrial Trifunctional Protein; Beta Subunit; 3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit; Beta-ketothiolase; HADHb elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HADHb. No significant cross-reactivity or interference between HADHb and analogues was observed.
• Sequence Length: 474

• Samples: Tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.053ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HADHb were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HADHb were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HADHb elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HADHb in human tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HADHb. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HADHb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HADHb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HADHb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504327
• NCBI GeneID: 3032
• NCBI Accession #: NP_000174.1
• NCBI GenBank Nucleotide #: NM_000183.2
• UniProt Accession #: P55084; O14969; Q53TA6; Q96C77; Q9H3F5; Q9T2V8; B2RB16; B4E2W0
• Molecular Weight: 48,880 Da
• NCBI Official Full Name: trifunctional enzyme subunit beta, mitochondrial isoform 1
• NCBI Official Synonym Full Names: hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta
• NCBI Official Symbol: HADHB
• NCBI Official Synonym Symbols: ECHB; MTPB; MSTP029; TP-BETA
• NCBI Protein Information: trifunctional enzyme subunit beta, mitochondrial
• UniProt Protein Name: Trifunctional enzyme subunit beta, mitochondrial
• Protein Family: Trifunctional enzyme
• UniProt Gene Name: HADHB

NCBI Description

This gene encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. Mutations in this gene result in trifunctional protein deficiency. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]