Immunofluorescence (IF)
(Immunofluorescence staining of CPLX2 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CPLX2 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).)
Rabbit anti-Human CPLX2 Monoclonal Antibody | anti-CPLX2 antibody
Recombinant Anti-CPLX2 Antibody, Rabbit Monoclonal
FC/FACS/FCM: 1:25-1:100
Immunofluorescence (IF)
(Immunofluorescence staining of CPLX2 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CPLX2 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).)
Immunofluorescence (IF)
(Immunofluorescence staining of CPLX2 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CPLX2 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CPLX2 expression on SHSY5Y cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human CPLX2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CPLX2 expression on SHSY5Y cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human CPLX2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
NCBI and Uniprot Product Information
NCBI Description
Proteins encoded by the complexin/synaphin gene family are cytosolic proteins that function in synaptic vesicle exocytosis. These proteins bind syntaxin, part of the SNAP receptor. The protein product of this gene binds to the SNAP receptor complex and disrupts it, allowing transmitter release. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CPLX2: Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in synaptic vesicle exocytosis. Also involved in mast cell exocytosis. Belongs to the complexin/synaphin family.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 5q35.2
Cellular Component: mast cell granule; cell soma; dendrite; synapse; cytosol
Molecular Function: syntaxin-1 binding; calcium-dependent protein binding
Biological Process: regulation of exocytosis; nervous system development; synaptic vesicle exocytosis; positive regulation of synaptic plasticity; mast cell degranulation; cell differentiation; vesicle docking during exocytosis
Research Articles on CPLX2
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Product Notes
The CPLX2 cplx2 (Catalog #AAA8105976) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CPLX2 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPLX2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the CPLX2 cplx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPLX2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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