Rabbit anti-Human MAGEF1 Polyclonal Antibody | anti-MAGEF1 antibody

MAGEF1, CT (Melanoma-associated Antigen F1, MAGE-F1 Antigen)

Cat. Num. AAA631028

• Gene Names: MAGEF1; MGC19617
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MAGEF1; Polyclonal Antibody; CT (Melanoma-associated Antigen F1; MAGE-F1 Antigen); Anti -MAGEF1; anti-MAGEF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAGEF1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MAGEF1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human MAGEF1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-MAGEF1 C-term Antibody is used in Western blot to detect MAGEF1 in A375 lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-MAGEF1 antibody

Melanoma-associated antigen (MAGE) are completely silent in normal tissues, with the exception of male germ cells, and, for some of them, placenta. These antigens ought to be strictly tumor specific, expressed in tumor cells of various histological types. Because of their specific expression on tumor cells, these antigens are of particular interest for antitumor immunotherapy. Genes of the MAGE family direct the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes. Though the function of MAGE is unknown, may play a role in embryonal development and tumor transformation or aspects of tumor progression.

Product Categories/Family for anti-MAGEF1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 29826296
• NCBI GeneID: 64110
• NCBI Accession #: NP_071432.2
• NCBI GenBank Nucleotide #: NM_022149.4
• UniProt Accession #: Q9HAY2; Q9H215
• Molecular Weight: 35,222 Da
• NCBI Official Full Name: melanoma-associated antigen F1
• NCBI Official Synonym Full Names: melanoma antigen family F, 1
• NCBI Official Symbol: MAGEF1
• NCBI Official Synonym Symbols: MGC19617
• NCBI Protein Information: melanoma-associated antigen F1; MAGE-F1 antigen; OTTHUMP00000210456
• UniProt Protein Name: Melanoma-associated antigen F1
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEF1
• UniProt Entry Name: MAGF1_HUMAN

NCBI Description

This intronless gene encodes a member of the MAGE superfamily. It is ubiquitously expressed in normal tissues and in tumor cells. This gene includes a microsatellite repeat in the coding region. [provided by RefSeq]

Uniprot Description

MAGE-F1: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Chromosomal Location of Human Ortholog: 3q13