CPLX2 recombinant protein

CPLX2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121014

• Gene Names: CPLX2; CPX2; Hfb1; 921-L; CPX-2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CPLX2; CPLX2 Protein; Human; Recombinant (His Tag); Human Complexin-2/CPLX2 Protein (His Tag); 921-L Protein; CPX-2 Protein; CPX2 Protein; Hfb1 Protein; complexin 2; CPLX2 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asp2-Lys134

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CPLX2 (Q6PUV4-1)(Asp 2-Lys 134) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CPLX2 recombinant protein

Background: Complexin-2 (CPLX2), a member of the complexin/synaphin family, is a soluble pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals. Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Complexins are unable to bind to monomeric SNARE proteins but bind with high affinity to ternary SNARE complexes and with lower affinity to target SNARE complexes. Complexin 1 (CX1) and complexin 2 (CX2) are presynaptic proteins that modulate neurotransmitter release and are used as markers of inhibitory and excitatory synapses, respectively. CPLX2 is localized in pre-synaptic terminals in mature brain. The G71-P89 region of CPLX2 is essential and sufficient for preferential axonal distribution. CPLX2 participates in the Ca(2+)-sensitive regulatory pathway for zymogen granule exocytosis. Complexin-2 is a key player in normal neurological function, and its downregulation could lead to changes in neurotransmitter release sufficient to cause significant behavioural abnormalities such as depression. It is involved in synaptogenesis and the modulation of neurotransmitter release.

References

Lee HJ, et al. (2005) Association study of polymorphisms in synaptic vesicle-associated genes, SYN2 and CPLX2, with schizophrenia. Behav Brain Funct. 1: 15.Salimi K, et al. (2008) Regulation of complexin 1 and complexin 2 in the developing human prefrontal cortex. Synapse. 62(4): 273-82.Kataoka M, et al. (2009) Identification of a minimal segment of complexin II essential for preferential distribution in axons. J Neurochem. 108(5): 1109-15.Glynn D, et al. (2010) Clorgyline-mediated reversal of neurological deficits in a Complexin 2 knockout mouse. Hum Mol Genet. 19(17): 3402-12.Falkowski MA, et al. (2010) Complexin 2 modulates vesicle-associated membrane protein (VAMP) 2-regulated zymogen granule exocytosis in pancreatic acini. J Biol Chem. 285(46): 35558-66.

NCBI and Uniprot Product Information

• NCBI GI #: 51316998
• NCBI GeneID: 10814
• UniProt Accession #: Q6PUV4; O09056; Q13329; Q28184; Q52M15; Q64386; B2RAG2
• Molecular Weight: 134
• NCBI Official Full Name: Complexin-2
• NCBI Official Synonym Full Names: complexin 2
• NCBI Official Symbol: CPLX2
• NCBI Official Synonym Symbols: CPX2; Hfb1; 921-L; CPX-2
• NCBI Protein Information: complexin-2; CPX II; synaphin 1; synaphin-1; complexin II
• UniProt Protein Name: Complexin-2
• Protein Family: Complexin
• UniProt Gene Name: CPLX2
• UniProt Synonym Gene Names: CPX II
• UniProt Entry Name: CPLX2_HUMAN

NCBI Description

Proteins encoded by the complexin/synaphin gene family are cytosolic proteins that function in synaptic vesicle exocytosis. These proteins bind syntaxin, part of the SNAP receptor. The protein product of this gene binds to the SNAP receptor complex and disrupts it, allowing transmitter release. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CPLX2: Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in synaptic vesicle exocytosis. Also involved in mast cell exocytosis. Belongs to the complexin/synaphin family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: mast cell granule; cell soma; dendrite; synapse; cytosol

Molecular Function: syntaxin-1 binding; calcium-dependent protein binding

Biological Process: regulation of exocytosis; nervous system development; synaptic vesicle exocytosis; positive regulation of synaptic plasticity; mast cell degranulation; cell differentiation; vesicle docking during exocytosis