Mouse COX IV Monoclonal Antibody | anti-COX4I1 antibody
Anti-COX IV Antibody (Monoclonal, 4G11)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Immunogen Affinity Purified
Synonyms
COX IV; Monoclonal Antibody; Anti-COX IV Antibody (Monoclonal; 4G11); Cytochrome c oxidase subunit 4 isoform 1; mitochondrial; cytochrome c oxidase subunit 4I1; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; COX4I1; COX4; anti-COX4I1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Applicable Applications for anti-COX4I1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)
Application Notes
WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
FC/FACS: 1-3ug/1x106 cells
IHC-P: 0.5-1ug/ml
FC/FACS: 1-3ug/1x106 cells
Immunogen
E Coli-derived human COX IV recombinant protein (Position: Q59-K169).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Relevant Detection Systems
Relevant Detection Systems
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
Relevant Detection Systems
Relevant Detection Systems
Relevant Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P).
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Related Product Information for anti-COX4I1 antibody
Description: Mouse IgG monoclonal antibody for COX IV detection. Tested with WB, IHC-P, FCM in Human; Mouse; Rat.
Background: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial is an enzyme that in humans is encoded by the COX4I1 gene. Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14.
Background: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial is an enzyme that in humans is encoded by the COX4I1 gene. Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14.
References
1. "Entrez Gene: COX4I1 cytochrome c oxidase subunit IV isoform 1". 2. Lomax MI, Welch MD, Darras BT, Francke U, Grossman LI (May 1990). "Novel use of a chimpanzee pseudogene for chromosomal mapping of human cytochrome c oxidase subunit IV". Gene. 86 (2): 209-16.
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
UniProt Protein Name
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
Protein Family
UniProt Gene Name
COX4I1
UniProt Synonym Gene Names
COX4; COX IV-1
UniProt Entry Name
COX41_HUMAN
Uniprot Description
COX4I1: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Ubiquitous. Belongs to the cytochrome c oxidase IV family.
Protein type: Oxidoreductase; Mitochondrial; EC 1.9.3.1; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation
Chromosomal Location of Human Ortholog: 16q24.1
Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; nucleus
Molecular Function: protein binding; cytochrome-c oxidase activity
Biological Process: cellular metabolic process; generation of precursor metabolites and energy; transmembrane transport; response to nutrient
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Product Notes
The COX4I1 cox4i1 (Catalog #AAA1752819) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-COX IV Antibody (Monoclonal, 4G11) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's COX IV can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS). WB: 0.1-0.5ug/ml IHC-P: 0.5-1ug/ml FC/FACS: 1-3ug/1x106 cells. Researchers should empirically determine the suitability of the COX4I1 cox4i1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX IV, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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