Mouse Galectin-3 Monoclonal Antibody | anti-LGALS3 antibody

Galectin-3 Antibody

Cat. Num. AAA415026

• Gene Names: LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: Galectin-3; Monoclonal Antibody; Galectin-3 Antibody; anti-LGALS3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Human, mouse and rat.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 250

• Applicable Applications for anti-LGALS3 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: 1:500 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5000.
• Immunogen: This protein has several names including macrophage galactose-specific lectin or MAC2, galactoside-binding protein or GALBP, or Galectin 3 or Gal 3. It is a highly conserved molecule about 30kDa in size. Galectin 3 is a member of a multigene family of 15 vertebrate lectins all of which share one or in some cases two "carbohydrate recognition domains" (CRD) which bind beta-galactoside a sugar moiety found on many cell surface proteins (1). Gal-3 is unusual in that it also includes, at the N terminus, multiple proline, tyrosine and glycine rich repeats which allows this molecule to oligomerize, so allowing high avidity binding to beta-galactoside. Gal-3 It is a multifunctional protein which is expressed both on the cell surface, cytoplasm and nucleus and which appears to function both in specific carbohydrate binding and in the regulation of mRNA splicing (1,2). Much evidence suggests that Gal-3 has an important role in the regulation of inflammatory responses and fibrogenesis (3). The HGNC name for this protein is LGALS3.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Testing Data #2

References

1. Haudek, K. C., Patterson, R. J., Wang, J. L. SR proteins and galectins: what's in a name? Glycobiology 20:1199-1207 (2011).2. Haudek, K. C., Spronk, K. J., Voss, P. G., Patterson, R. J., Wang, J. L., Arnoys, E. J. Dynamics of galectin-3 in the nucleus and cytoplasm. Biochim. Biophys. Acta. 1800:181-189 (2011).3. Henderson, N. C., Sethi, T. The regulation of inflammation by galectin-3. Immunological Reviews 230:160 -171 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 2385452
• NCBI GeneID: 3958
• UniProt Accession #: P17931; Q16005; Q6IBA7; Q96J47; B2RC38
• Molecular Weight: 26,152 Da
• NCBI Official Full Name: galectin-3
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 3
• NCBI Official Symbol: LGALS3
• NCBI Official Synonym Symbols: L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• NCBI Protein Information: galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35
• UniProt Protein Name: Galectin-3
• Protein Family: Galectin
• UniProt Gene Name: LGALS3
• UniProt Synonym Gene Names: MAC2; Gal-3; CBP 35; GALBP
• UniProt Entry Name: LEG3_HUMAN

NCBI Description

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

Uniprot Description

Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis

By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Ref.15 Ref.16 Ref.17

Subunit structure: Probably forms homo- or heterodimers. Interacts with DMBT1

By similarity. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. Ref.14 Ref.15

Subcellular location: Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface. Ref.5 Ref.15 Ref.17

Tissue specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.

Sequence similarities: Contains 1 galectin domain.