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Western Blot (WB)
(Western blot analysis of SARS-CoV-2 spike S2 RBD recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 1ug/ml, Lane 2: 2ug/ml, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Rabbit anti-Viral COVID 19 Spike Protein Rbd Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
Rabbit anti SARS-CoV-2 Spike Protein Rbd
Reactivity
Viral
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Rabbit polyclonal antibody purified by affinity chromatography
Synonyms
COVID 19 Spike Protein Rbd Coronavirus; Polyclonal Antibody; Rabbit anti SARS-CoV-2 Spike Protein Rbd; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; SARS-CoV-2 Spike Protein Rbd antibody; SARS-CoV-2 SPIKE PROTEIN RBD; anti-COVID-19 antibody
Host
Rabbit
Reactivity
Viral
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Rabbit polyclonal antibody purified by affinity chromatography
Form/Format
Phosphate buffered saline
0.02% Sodium Azide (NaN3)
Purified IgG - liquid
0.02% Sodium Azide (NaN3)
Purified IgG - liquid
Concentration
1.0mg/ml (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
ELISA: 1000ng/ml to 8ng/ml
IF: 20ug/ml
WB: 5ug/ml to 2ug/ml
IF: 20ug/ml
WB: 5ug/ml to 2ug/ml
Immunogen
Peptide corresponding to 19 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein RBD.
The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Spike protein RBD.
The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Spike protein RBD.
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Western Blot (WB)
(Western blot analysis of SARS-CoV-2 spike S2 RBD recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 1ug/ml, Lane 2: 2ug/ml, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Western Blot (WB)
(Western blot analysis of SARS-CoV-2 spike S2 RBD recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 1ug/ml, Lane 2: 2ug/ml, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Immunocytochemistry (ICC)
(Immunocytochemistry of HEK293 cells transfected with SARS-CoV-2 spike protein, fixed with PFA and probed using Rabbit anti SARS-CoV-2 spike protein RBD antibody (20mg/ml, MBS226235) (green). Nucleus labeled with DAPI (blue).)
Immunocytochemistry (ICC)
(Immunocytochemistry of HEK293 cells transfected with SARS-CoV-2 spike protein, fixed with PFA and probed using Rabbit anti SARS-CoV-2 spike protein RBD antibody (20mg/ml, MBS226235) (green). Nucleus labeled with DAPI (blue).)
Western Blot (WB)
(Western blot analysis of HEK293 cells untreated or transfected with SARS-CoV-2 spike protein and probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (1/1000, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Western Blot (WB)
(Western blot analysis of HEK293 cells untreated or transfected with SARS-CoV-2 spike protein and probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (1/1000, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Western Blot (WB)
(Western blot analysis of SARS-CoV-2 spike RBD+SD1 recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 1ug/ml, Lane 2: 2ug/ml, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Western Blot (WB)
(Western blot analysis of SARS-CoV-2 spike RBD+SD1 recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 1ug/ml, Lane 2: 2ug/ml, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
ELISA (EIA)
(Direct ELISA using SARS-CoV-2 spike RBD+SD1 recombinant protein as the coating antigen and Rabbit anti SARS-CoV-2 spike protein RBD antibody (MBS226235) as the capture antibody. Detection range is from 8ng/ml to 1000ng/ml.)
ELISA (EIA)
(Direct ELISA using SARS-CoV-2 spike RBD+SD1 recombinant protein as the coating antigen and Rabbit anti SARS-CoV-2 spike protein RBD antibody (MBS226235) as the capture antibody. Detection range is from 8ng/ml to 1000ng/ml.)
Western Blot (WB)
(Western blot analysis of SARS-CoV-2 spike RBD+SD1+SD2 recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 1ug/ml, Lane 2: 2ug/ml, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Western Blot (WB)
(Western blot analysis of SARS-CoV-2 spike RBD+SD1+SD2 recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 1ug/ml, Lane 2: 2ug/ml, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Western Blot (WB)
(Western blot analysis of SARS-CoV-2 spike S1 recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 2.5ug/ml, Lane 2: 5ug/ml, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Western Blot (WB)
(Western blot analysis of SARS-CoV-2 spike S1 recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 2.5ug/ml, Lane 2: 5ug/ml, MBS226235). Arrow points to SARS-CoV-2 spike protein.)
Related Product Information for anti-COVID-19 antibody
Rabbit anti SARS-CoV-2 spike protein RBD antibody recognizes spike glycoprotein, also known as S glycoprotein, peplomer protein and E2.
Human coronaviruses are pathogens that can cause severe respiratory tract infections. SARS-CoV-2 is a novel human coronavirus characterized in 2020, and causes "coronavirus disease 2019" or "COVID-19" (Xu et al. 2020). The spike glycoproteins of coronaviruses are incorporated into the viral envelope and promote entry into cells (Walls et al. 2020). The spike glycoprotein of SARS-CoV-2 is comprised of two subunits called S1 and S2. The S1 protein binds to a receptor on human cells, and the transmembrane unit S2 helps fuse the viral membrane with a cellular membrane (Hoffmann et al. 2020). Specifically, both SARS-CoV and SARS-CoV-2 spike proteins contain a receptor-binding domain (RBD) that recognize the same receptor, ACE2, on human cells (Shang et al. 2020). This antibody recognizes the RBD of the SARS-CoV-2 spike protein.
Human coronaviruses are pathogens that can cause severe respiratory tract infections. SARS-CoV-2 is a novel human coronavirus characterized in 2020, and causes "coronavirus disease 2019" or "COVID-19" (Xu et al. 2020). The spike glycoproteins of coronaviruses are incorporated into the viral envelope and promote entry into cells (Walls et al. 2020). The spike glycoprotein of SARS-CoV-2 is comprised of two subunits called S1 and S2. The S1 protein binds to a receptor on human cells, and the transmembrane unit S2 helps fuse the viral membrane with a cellular membrane (Hoffmann et al. 2020). Specifically, both SARS-CoV and SARS-CoV-2 spike proteins contain a receptor-binding domain (RBD) that recognize the same receptor, ACE2, on human cells (Shang et al. 2020). This antibody recognizes the RBD of the SARS-CoV-2 spike protein.
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The COVID-19 (Catalog #AAA226235) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti SARS-CoV-2 Spike Protein Rbd reacts with Viral and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike Protein Rbd Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). ELISA: 1000ng/ml to 8ng/ml IF: 20ug/ml WB: 5ug/ml to 2ug/ml. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike Protein Rbd Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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