FACS Assay
(FACS assay showed 2019-nCoV Spike protein RBD (MBS515722) and 2019-nCoV Spike protein S1 (MBS515719) can bind to ACE2 overexpressing cells (Panel A and B respectively). ACE2 overexpressing cells were stained with 2019-nCoV Spike protein RBD or S1, followed by anti-Spike protein antibody and fluorescence-conjugated secondary antibody.)
COVID 19 Spike Coronavirus Recombinant Antibody | COVID-19 recombinant antibody
Anti-2019nCoV Spike Protein, Recombinant Human Antibody
Reactivity
SARS-CoV spike protein
Applications
ELISA, Flow Cytometry, Functional Assay
Synonyms
COVID 19 Spike Coronavirus; Monoclonal Recombinant Antibody; Anti-2019nCoV Spike Protein; Recombinant Human Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike Protein; Anti-SARS-CoV-2 Spike Protein Antibody; COVID-19 recombinant antibody
Host
Human
Reactivity
SARS-CoV spike protein
Clonality
Monoclonal
Isotype
IgG
Specificity
Recognizes the 2019-nCoV spike protein
Form/Format
PBS + 0.02% soidum azide.
Concentration
0.5 mg/mL (varies by lot)
Applicable Applications for COVID-19 recombinant antibody
ELISA, Flow Cytometry (FACS)
Application Notes
Ideal working dilutions for each application should be empirically determined by the investigator.
Preparation and Storage
Store at -20°C for 1 year from date of receipt. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
FACS Assay
(FACS assay showed 2019-nCoV Spike protein RBD (MBS515722) and 2019-nCoV Spike protein S1 (MBS515719) can bind to ACE2 overexpressing cells (Panel A and B respectively). ACE2 overexpressing cells were stained with 2019-nCoV Spike protein RBD or S1, followed by anti-Spike protein antibody and fluorescence-conjugated secondary antibody.)
FACS Assay
(FACS assay showed 2019-nCoV Spike protein RBD (MBS515722) and 2019-nCoV Spike protein S1 (MBS515719) can bind to ACE2 overexpressing cells (Panel A and B respectively). ACE2 overexpressing cells were stained with 2019-nCoV Spike protein RBD or S1, followed by anti-Spike protein antibody and fluorescence-conjugated secondary antibody.)
Biological Activity
(Binding of anti-2019nCoV spike protein antibody (MBS515718) to 2019nCoV RBD (MBS515722) determined by ELISA.)
Biological Activity
(Binding of anti-2019nCoV spike protein antibody (MBS515718) to 2019nCoV RBD (MBS515722) determined by ELISA.)
Related Product Information for COVID-19 recombinant antibody
The severe acute respiratory syndrome related novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). The spike glycoprotein (S) of coronavirus belongs to the type I transmembrane protein containing two subunits, S1 and S2 (2), which is also known to be the key component to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) (3). A receptor binding domain (RBD) of S1 can recognize the cell surface receptor and the mutation of RBD could cause higher motility rate (3).
Product Categories/Family for COVID-19 recombinant antibody
References
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89. 2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30. 3. Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor.
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Product Notes
The COVID-19 (Catalog #AAA515718) is a Recombinant Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-2019nCoV Spike Protein, Recombinant Human Antibody reacts with SARS-CoV spike protein and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA, Flow Cytometry (FACS). Ideal working dilutions for each application should be empirically determined by the investigator. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike Coronavirus, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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