Rabbit anti-Human Connexin 40 Polyclonal Antibody | anti-GJA5 antibody

Connexin 40, NT (Gap Junction alpha-5 Protein, Connexin-40, Cx40, GJA5)

Cat. Num. AAA648147

• Gene Names: GJA5; CX40; ATFB11
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Connexin 40; Polyclonal Antibody; NT (Gap Junction alpha-5 Protein; Connexin-40; Cx40; GJA5); Anti -Connexin 40; anti-GJA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GJA5.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-GJA5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the N-terminal region of human GJA5 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-GJA5 N-term Pab in Placenta lysate. GJA5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-GJA5 antibody

Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.

Product Categories/Family for anti-GJA5 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1220303
• NCBI GeneID: 2702
• UniProt Accession #: P36382; Q5T3B6; Q5U0N6
• Molecular Weight: 40,380 Da
• NCBI Official Full Name: connexin 40
• NCBI Official Synonym Full Names: gap junction protein, alpha 5, 40kDa
• NCBI Official Symbol: GJA5
• NCBI Official Synonym Symbols: CX40; ATFB11
• NCBI Protein Information: gap junction alpha-5 protein; connexin 40
• UniProt Protein Name: Gap junction alpha-5 protein
• Protein Family: Gap junction alpha-5 protein
• UniProt Gene Name: GJA5
• UniProt Synonym Gene Names: Cx40
• UniProt Entry Name: CXA5_HUMAN

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subunit structure: A connexon is composed of a hexamer of connexins.

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.

Involvement in disease: Familial atrial standstill (FAS) [MIM:108770]: Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm.Note: The disease may be caused by mutations affecting distinct genetic loci, including the gene represented in this entry. A rare GJA5 genotype has been detected in combination with a mutation in SCN5A in a large family with atrial standstill. Ref.8Familial atrial fibrillation 11 (ATFB11) [MIM:614049]: A familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9

Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.