Western Blot (WB)
(Western blot analysis of (A) mouse thalamus homogenates and (B) connexin47 cDNA-transfected HeLa cells using C7859-01)
Mouse anti-Mouse Connexin 47 Monoclonal Antibody | anti-Gjc2 antibody
Connexin 47 (MaxLight 650)
Gene Names
Gjc2; Cx47; Gja12; B230382L12Rik
Reactivity
Mouse
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Highly purified from mouse ascites by Protein A Chromatography
Synonyms
Connexin 47; Monoclonal Antibody; Connexin 47 (MaxLight 650); anti-Gjc2 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4H308
Specificity
Reacts with the mouse Connexin 47 protein. On Western blots of mouse thalamus homogenates and Connexin 47-transfected HeLa cells, it identified a band at ~47kD. Reactivity was confirmed in Western blots with mouse thalamus lysates. Mouse Connexin 47-transfected HeLa cells were used in both Immunoprecipitation and Western blot assays. This antibody was also used in immunofluorescence assays using paraformaldehyde-fixed mouse brain. The expected staining pattern is punctate labeling of the oligodendrocytes in the brain.
Purity/Purification
Highly purified from mouse ascites by Protein A Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight650.
Applicable Applications for anti-Gjc2 antibody
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Synthetic peptide derived from the C-terminal region of the mouse Connexin 47 protein.
Conjugate
MaxLight650
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Western Blot (WB)
(Western blot analysis of (A) mouse thalamus homogenates and (B) connexin47 cDNA-transfected HeLa cells using C7859-01)
Western Blot (WB)
(Western blot analysis of (A) mouse thalamus homogenates and (B) connexin47 cDNA-transfected HeLa cells using C7859-01)
Product Categories/Family for anti-Gjc2 antibody
References
1. Bennet MV, et al. Neuron 6(3): 305-320, 1991. 2. Kumar NM & Gilula NB. Cell 84(3): 381-388, 1996. 3. Kuraoka A. J Histochem Cytochem 41(7): 971-980, 1993. 4. Manthey D, et al. J Biol Chem 274(21): 14716-14723, 1999. 5. Rozental R, et al. Brain Res Brain Res Rev 32(1): 11-15, 2000. 6. Schwartz HJ, et al. Somat Cell Mol Genet 18(4): 351-359, 1992. 7. Sutor B, et al. Cereb Cortex 10(7): 684-697, 2000. 8. Teubner B, et al. J Neurosci 21(4):1117-1126, 2001. 9. White TW, et al. Annu Rev Physiol 61: 283-310, 1999. 10. Wilgenbus KK, et al. Int J Cancer 51(4): 522-529, 1992. 11. Willecke K, et al. Novartis Found Symp 219: 76-88, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,008 Da
NCBI Official Full Name
gap junction gamma-2 protein
NCBI Official Synonym Full Names
gap junction protein, gamma 2
NCBI Official Symbol
Gjc2
NCBI Official Synonym Symbols
Cx47; Gja12; B230382L12Rik
NCBI Protein Information
gap junction gamma-2 protein; connexin-47; connexin 47 type A; connexin 47 type C; connexin 47 type D; gap junction protein, chi 2; gap junction alpha-12 protein; gap junction membrane channel protein alpha 12
UniProt Protein Name
Gap junction gamma-2 protein
Protein Family
UniProt Gene Name
Gjc2
UniProt Synonym Gene Names
Gja12; Cx47
UniProt Entry Name
CXG2_MOUSE
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Product Notes
The Gjc2 gjc2 (Catalog #AAA6259819) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Connexin 47 (MaxLight 650) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 47 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the Gjc2 gjc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 47, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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