Mouse Complement C5b Neo-epitope Monoclonal Antibody
Complement C5b Neo-epitope, Human
Reactivity
Bovine, Goat, Human, Monkey, Porcine
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Complement C5b Neo-epitope; Monoclonal Antibody; Human; Anti -Complement C5b Neo-epitope; anti-Complement C5b Neo-epitope antibody
Host
Mouse
Reactivity
Bovine, Goat, Human, Monkey, Porcine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5F208
Specificity
Recognizes human C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5 (See C7850-23). Normal fresh and activated serum subjected to SDS-PAGE Western Blot (nonreduced), visualizes reactivity with the SDS-denatured C5 (200kD) abd C5b (185kD), the latter together with a faint cleavage product (60kD) only after activation.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a lyophilized powder. Reconstitute with 1ml sterile dH2O.
Applicable Applications for anti-Complement C5b Neo-epitope antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Immunohistochemistry (frozen): 1:10. Incubation time 1hr at RT.
Dilution: Immunohistochemistry (frozen): 1:10. Incubation time 1hr at RT.
Immunogen
Activated human complement component C5.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Complement C5b Neo-epitope antibody
C5 is involved in the activation of the lythic pathway within the complement system which is an important factor in innate immunity. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5 into C5a and C5b. C5b initiates the assembly of the membrane attack complex (MAC) that mediates cytolysis.
Product Categories/Family for anti-Complement C5b Neo-epitope antibody
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Product Notes
The Complement C5b Neo-epitope (Catalog #AAA600262) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Complement C5b Neo-epitope, Human reacts with Bovine, Goat, Human, Monkey, Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's Complement C5b Neo-epitope can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: Immunohistochemistry (frozen): 1:10. Incubation time 1hr at RT. Researchers should empirically determine the suitability of the Complement C5b Neo-epitope for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement C5b Neo-epitope, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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