Mouse anti-Human Complement C5, C5a Monoclonal Antibody | anti-C5 antibody

Complement C5, C5a

Cat. Num. AAA632494

• Gene Names: C5; CPAMD4; FLJ17816; FLJ17822; MGC142298
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Complement C5; C5a; Monoclonal Antibody; Anti -Complement C5; anti-C5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 6A533
• Specificity: Recognizes an epitope of human C5 and C5a. Capable in inhibiting the binding of C5a to the C5a receptor through a competitive mechanism. Does not block the cleavage of C5 into C5a and C5b.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA.

• Applicable Applications for anti-C5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot., Recommended Dilutions:, ELISA: Used as detection antibody., Western Blot: 1:50-1:1000. Incubate for 2 hours on nitrocellulose., Optimal dilutions to be determined by the researcher., Storage and Stability:, May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Immunogen: BALB/c mice were immunized with human C5.
• Preparation and Storage: -20 degree C

Testing Data

(Immuno assay experiment: MBS632494 was used as a detection antibody in different concentrations.)

Related Product Information for anti-C5 antibody

The complement system is composed of over 30 proteins, activated in response to tissue injury, invading pathogens or other foreign surfaces. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5. C5a was first described as a cleavage product of C5 with chemotactic and anaphylatoxic properties. Further characterization revealed that C5a is an essential part of the innate immune response and evidence now suggests that it may also play a role in adaptive immunity. Complement fragment C5a is a 74aa pro-inflammatory polypeptide. C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77), expressed ubiquitously on a wide variety of cells but particularly on the surface of immune cells like macrophages, neutrophils and T cells. The former is a well-established receptor that initiates G-protein-coupled signaling via mitogen-activated protein kinase pathways, thereby by inducing synthesis of cytokines. Its in vivo blockade greatly reduces inflammatory injury. Much less is known about C5L2, occupancy of which by C5a does not initiate increased intracellular Ca(2+). The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases. C5a is also implicated in non-immunological functions associated with developmental biology, CNS development and neurodegeneration, tissue regeneration, and haematopoiesis.

Product Categories/Family for anti-C5 antibody

Antibodies; Abs to Complement

NCBI and Uniprot Product Information

• NCBI GI #: 38016947
• NCBI GeneID: 727
• NCBI Accession #: NP_001726.2
• NCBI GenBank Nucleotide #: NM_001735.2
• UniProt Accession #: P01031; Q14CJ0; Q27I61; Q59GS8
• Molecular Weight: 188,305 Da
• NCBI Official Full Name: complement C5 preproprotein
• NCBI Official Synonym Full Names: complement component 5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: CPAMD4; FLJ17816; FLJ17822; MGC142298
• NCBI Protein Information: complement C5; OTTHUMP00000022001; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
• UniProt Protein Name: Complement C5
• Protein Family: Complement C5
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: CPAMD4
• UniProt Entry Name: CO5_HUMAN

NCBI Description

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq]

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q33-q34

Cellular Component: membrane attack complex; extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; endopeptidase inhibitor activity; C5a anaphylatoxin chemotactic receptor binding; receptor binding

Biological Process: positive regulation of chemotaxis; activation of MAPK activity; in utero embryonic development; cytolysis; complement activation, alternative pathway; glucose homeostasis; chemotaxis; leukocyte migration during inflammatory response; complement activation; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of complement activation; response to stress; innate immune response; negative regulation of dopamine secretion; inflammatory response; complement activation, classical pathway

Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To