Aminopeptidase A Active Protein | ENPEP active protein

Aminopeptidase A Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122523

• Gene Names: Enpep; APA; Ly51; Ly-51; Bp-1/6C3; 6030431M22Rik
• Purity: >91% as determined by SDS-PAGE
• Synonyms: Aminopeptidase A; Aminopeptidase A Protein; Mouse; Recombinant (His Tag); Mouse ENPEP/Aminopeptidase A Protein (His Tag); 6030431M22Rik Protein; APA Protein; Bp-1/6C3 Protein; ENPEP Protein; Ly-51 Protein; Ly51 Protein; glutamyl aminopeptidase; ENPEP active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >91% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Arg41-Pro945

• Species: Mouse
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin. The specific activity is >200pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of mouse ENPEP (P16406)(Arg41-Pro945) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for ENPEP active protein

Background: ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.

References

Speth RC, et al. (2008) The significance of brain aminopeptidases in the regulation of the actions of angiotensin peptides in the brain. Heart Fail Rev. 13(3):299-309.Rose JE, et al. (2010) Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Mol Med. 16(7-8):247-53.Pa©rez I, et al. (2009) Increased APN/CD13 and acid aminopeptidase activities in head and neck squamous cell carcinoma. Head Neck. 31(10):1335-40.

NCBI and Uniprot Product Information

• NCBI GI #: 6679653
• NCBI GeneID: 13809
• NCBI Accession #: NP_031960.1
• NCBI GenBank Nucleotide #: NM_007934.3
• UniProt Accession #: P16406
• Molecular Weight: 107,956 Da
• NCBI Official Full Name: glutamyl aminopeptidase
• NCBI Official Synonym Full Names: glutamyl aminopeptidase
• NCBI Official Symbol: Enpep
• NCBI Official Synonym Symbols: APA; Ly51; Ly-51; Bp-1/6C3; 6030431M22Rik
• NCBI Protein Information: glutamyl aminopeptidase
• UniProt Protein Name: Glutamyl aminopeptidase
• Protein Family: Aminopeptidase
• UniProt Gene Name: Enpep
• UniProt Synonym Gene Names: EAP; AP-A
• UniProt Entry Name: AMPE_MOUSE

Uniprot Description

ENPEP: Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells. Belongs to the peptidase M1 family.

Protein type: Protease; EC 3.4.11.7; Membrane protein, integral

Cellular Component: apical part of cell; apical plasma membrane; brush border; cytoplasmic vesicle; external side of plasma membrane; integral to membrane; lysosomal membrane; membrane; plasma membrane; vesicle membrane

Molecular Function: aminopeptidase activity; hydrolase activity; metal ion binding; metallopeptidase activity; peptidase activity; peptide binding; zinc ion binding

Biological Process: angiogenesis; aromatic compound metabolic process; cell migration; cell proliferation; peptide catabolic process; proteolysis; regulation of blood pressure; regulation of systemic arterial blood pressure by renin-angiotensin