Rat anti-Mouse CMKLR1 Monoclonal Antibody | anti-CMKLR1 antibody

Anti-CMKLR1 [BZ194]

Cat. Num. AAA488615

• Gene Names: Cmklr1; DEZ; Gpcr27; ChemR23; mcmklr1
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot, ELISA, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: CMKLR1; Monoclonal Antibody; Anti-CMKLR1 [BZ194]; Chemerin receptor; CHEMERINR; chemokine receptor-like 1; Chemokine-like receptor 1; ChemR23; DEZ; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; orphan G-protein coupled receptor; Dez; Resolvin E1 receptor; RVER1; Gpcr27; MGC126105; MGC126106; anti-CMKLR1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a, kappa
• Clone Number: BZ194
• Specificity: This antibody is specific for murine serpentine receptor mCMKLR1 (aka ChemR23, DEZ). mCMKLR1 is a novel protein possessing high homology with members of the chemoattractant receptor family, and binds the chemoattractant chemerin. Selective mCMKLR1 expression has been demonstrated by mouse macrophages ex vivo, and by dendritic cell precursors cultured in vitro.
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CMKLR1 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Functional Studies
• Application Notes: The anti-mCMKLR1 BZ194 generates a robust staining signal when used in flow cytometry applications with mCMKLR1+ cells (Zabel et al., 2006). This antibody, together with other mAbs directed against chemokine receptors, could be used to determine the leukocyte expression profile of receptors during homeostasis or inflammation, to elucidate the role of various receptors in coordinating the immune response and in leukocyte development, and to identify other proteins interacting with the chemokine receptor. In particular, this antibody has been used to evaluate the functional responses of mCMKLR1-expressing cells to chemerin (Zabel et al., 2006), to demonstrate the esssential role of natural killer cells in resolution of antigen-induced inflammation in mice (Anuforo et al., 2018), to indicate a potential role for chemerin and CMKLR1 in the regulation of inflammatory responses in the tumor microenvironment (Rama et al, 2011), to visualise immediate immune responses to pioneer metastatic cells in the lung (Headley et al., 2016), and to investigate the regulatory role of chemerin in lung tumorigenesis and cancer-associated inflammation (Unver et al, 2018).
• PMID: 16863918
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed RAW264.7 cells with anti-CMKLR1 antibody BZ194 (MBS488614) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of BZ194 (MBS488614) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488614, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

Related Product Information for anti-CMKLR1 antibody

Recombinant monoclonal antibody to CMKLR1. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BZ194.
This antibody was raised by immunising Wistar Furth rats with the mCMKLR1 peptide/KLH conjugate. The immunizing amino-terminal mCMKLR1 peptide with the sequence NH2-DSGIYDDEYSDGFGYFVDLEEASPWC-COOH, corresponding to residues residues 8-32 of mCMKLR1, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

Product Categories/Family for anti-CMKLR1 antibody

immunology; cell signalling; immune system profiling; immunoassay; immunocytometry; chemokine; chemokine receptor; chemokine targets

References

Zabel et al. Chemokine-like receptor 1 expression by macrophages in vivo: regulation by TGF-beta and TLR ligands. Exp Hematol. 2006 Aug;34(8):1106-14.
Note on publication: Describe the original generation of this antibody and its subsequent use in FACS to assess mCMKLR1 expression by mouse leukocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 33469077
• NCBI GeneID: 14747
• NCBI Accession #: NP_032179.1
• NCBI GenBank Nucleotide #: NM_008153.3
• UniProt Accession #: P97468
• Molecular Weight: 41,815 Da
• NCBI Official Full Name: chemokine-like receptor 1
• NCBI Official Synonym Full Names: chemokine-like receptor 1
• NCBI Official Symbol: Cmklr1
• NCBI Official Synonym Symbols: DEZ; Gpcr27; ChemR23; mcmklr1
• NCBI Protein Information: chemokine-like receptor 1; chemerin receptor; chemokine receptor-like 1; G-protein coupled receptor 27; G-protein coupled receptor DEZ
• UniProt Protein Name: Chemokine-like receptor 1
• UniProt Gene Name: Cmklr1
• UniProt Synonym Gene Names: Dez; Gpcr27
• UniProt Entry Name: CML1_MOUSE

Uniprot Description

Function: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B

By similarity. Ref.3 Ref.4

Subcellular location: Cell membrane; Multi-pass membrane protein Ref.4.

Tissue specificity: Highly expressed in immature plasmacytoid dendritic cells (DCs) and at lower levels in myeloid DCs, macrophages, and NK cells. Expressed on macrophages isolated from differents tissues, including peritoneal cavities, pleural cavities and spleen. Ref.4 Ref.5

Developmental stage: Expressed during bone development and in adult parathyroid glands. Expressed at day E11 in the caudal part of the tongue and the umbilical cord and the expression in the tongue was maintained throughout adulthood. Expression increases in bone and cartilaginous forming zones of embryo up to stage E14.5 and at E16.5 expression is seen in the lung. Ref.1

Induction: Down-regulated by bacterial lipopolysaccharide (LPS), IFN-alpha and TNF on macrophages. Ref.4

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.