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Immunohistochemistry (IHC) (Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

Rabbit CKMB/Creatine Kinase MB Monoclonal Antibody | anti-CKMB antibody

Rabbit CKMB/Creatine Kinase MB Monoclonal Antibody

Reactivity
Human
Predicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
CKMB/Creatine Kinase MB; Monoclonal Antibody; Rabbit CKMB/Creatine Kinase MB Monoclonal Antibody; Mouse Monoclonal (IgG1; k) to Human CKMB/Creatine Kinase MB; anti-CKMB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted: Mouse, Rat
Clonality
Monoclonal
Specificity
Endogenous levels of human, mouse and rat Creatine kinase M and Creatine kinase B. Positive Control: HEK293, SH-SY5Y, mouse brain and rat brain.
Purity/Purification
Immunoaffinity Purified
Form/Format
Lyophilized from PBS, pH 7.4, 0.02% sodium azide.
Applicable Applications for anti-CKMB antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 10 ug/ml
WB: 0.5-1 ug/ml
Immunogen
KLH-coupled synthetic peptide within residues 300-350 of human Creatine kinase M (Swiss Prot: P06732) and Creatine kinase B (Swiss Prot: P12277)
Conjugation
Unconjugated
Epitope
aa300-350
Resuspension Liquid
Distilled water
Preparation and Storage
Store lyophilized at -20 degree C.
The reconstituted product can be stored for short term at 4 degree C or long term at -20 degree C or below.
Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC) (Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot of cell and tissue lysates using 1 ug/ml Rabbit Anti-Creatine kinase MB Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: Creatine kinase M 43 KD Creatine kinase B 43 KD. Observed Size: Creatine kinase M 43 KD Creatine kinase B 43 KD.)

Western Blot (WB) (Western blot of cell and tissue lysates using 1 ug/ml Rabbit Anti-Creatine kinase MB Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: Creatine kinase M 43 KD Creatine kinase B 43 KD. Observed Size: Creatine kinase M 43 KD Creatine kinase B 43 KD.)
Related Product Information for anti-CKMB antibody
cytosolic CK enzymes consist of two subunits, which can be either B(brain type) or M (muscle type). there are, the refore, three differentisoenzymes: CK-MM, CK-BB and CK-MB. the genes for these subunits arelocated on different chromosomes: B on 14q32 and M on 19q13. In additionto those three cytosolic CK isoforms, there are two mitochondrialcreatine kinase isoenzymes, the ubiquitous and sarcomeric form.

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Product Notes

The CKMB (Catalog #AAA2401741) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit CKMB/Creatine Kinase MB Monoclonal Antibody reacts with Human Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CKMB/Creatine Kinase MB can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 10 ug/ml WB: 0.5-1 ug/ml. Researchers should empirically determine the suitability of the CKMB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CKMB/Creatine Kinase MB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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