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Western Blot (WB) (Western blot analysis of CKB (arrow) using rabbit polyclonal CKB-C254. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CKB gene.)

Rabbit anti-Human, mouse Creatine Kinase B Polyclonal Antibody | anti-CKB antibody

Creatine Kinase B Antibody (C-term)

Gene Names
CKB; BCK; B-CK; CKBB; HEL-211; HEL-S-29
Reactivity
Human, mouse
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Creatine Kinase B; Polyclonal Antibody; Creatine Kinase B Antibody (C-term); Creatine kinase B-type; B-CK; Creatine kinase B chain; CKB; CKBB; anti-CKB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Creatine Kinase B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-270 amino acids from the C-terminal region of human Creatine Kinase B.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
240-270
Sequence Length
381
Applicable Applications for anti-CKB antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of CKB (arrow) using rabbit polyclonal CKB-C254. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CKB gene.)

Western Blot (WB) (Western blot analysis of CKB (arrow) using rabbit polyclonal CKB-C254. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CKB gene.)

Western Blot (WB)

(The anti-CKB Pab is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2).)

Western Blot (WB) (The anti-CKB Pab is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2).)
Related Product Information for anti-CKB antibody
Creatine Kinase (CK) is a dimeric enzyme composed of either M- or B-type subunits. The resulting isoenzymes are expressed at varying levels in different tissues. CK-BB, a cytoplasmic predominantly found in brain tissues, participates in energy homeostasis, reversibly catalyzing the transfer of a phosphate group between ATP and target proteins such as a creatine phosphate. CK-BB exists in normally neglible amounts in the serum of adults, overexpression of CK-BB is associated with cancers of the breast, ovary, prostate, colon, and in small-cell lung cancer. Global assessment of changes in serum levels of CK-BB, CK-MB and CK-MM, are used as a marker for myocardial infarction.
Product Categories/Family for anti-CKB antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42644
NCBI Official Full Name
creatine kinase B-type
NCBI Official Synonym Full Names
creatine kinase, brain
NCBI Official Symbol
CKB
NCBI Official Synonym Symbols
BCK; B-CK; CKBB; HEL-211; HEL-S-29
NCBI Protein Information
creatine kinase B-type
UniProt Protein Name
Creatine kinase B-type
Protein Family
UniProt Gene Name
CKB
UniProt Synonym Gene Names
CKBB
UniProt Entry Name
KCRB_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

CKB: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Kinase, other; Amino Acid Metabolism - arginine and proline; EC 2.7.3.2

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: mitochondrion; cytosol

Molecular Function: protein binding; creatine kinase activity; ATP binding

Biological Process: substantia nigra development; phosphorylation; cellular chloride ion homeostasis; creatine metabolic process

Research Articles on CKB

Similar Products

Product Notes

The CKB ckb (Catalog #AAA9201269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 240-270. The Creatine Kinase B Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Creatine Kinase B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the CKB ckb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Creatine Kinase B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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