Testing Data
(Fig.1: FACS analysis of BOSC23 cells using NJ-5C9 Cat.# GM-0909. BOSC23 cells were transiently transfected with an expression vector encoding either CGA (red curve) or an irrelevant protein (control transfectant: black curve). Binding of NJ-5C9 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CGA transfected cells.)
Mouse anti-Human Chromogranin A Monoclonal Antibody | anti-CHGA antibody
anti-human Chromogranin A (CGA) monoclonal antibody
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using NJ-5C9 Cat.# GM-0909. BOSC23 cells were transiently transfected with an expression vector encoding either CGA (red curve) or an irrelevant protein (control transfectant: black curve). Binding of NJ-5C9 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CGA transfected cells.)
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using NJ-5C9 Cat.# GM-0909. BOSC23 cells were transiently transfected with an expression vector encoding either CGA (red curve) or an irrelevant protein (control transfectant: black curve). Binding of NJ-5C9 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CGA transfected cells.)
Testing Data #2
(Fig.2: CGE analysis of purified NJ-5C9 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified NJ-5C9 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Testing Data #2
(Fig.2: CGE analysis of purified NJ-5C9 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified NJ-5C9 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Deftos LJ (1991). Chromogranin A: its role in endocrine function and as an endocrine and neuroendocrine tumor marker. Endocr Rev; 12(2):181-7.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells.
Protein type: Secreted; Secreted, signal peptide; Vesicle; Cell adhesion
Chromosomal Location of Human Ortholog: 14q32
Cellular Component: perinuclear region of cytoplasm; extracellular region; secretory granule
Biological Process: regulation of blood pressure
Research Articles on CHGA
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Product Notes
The CHGA chga (Catalog #AAA468047) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human Chromogranin A (CGA) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Chromogranin A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry(IHC). ELISA: 1:200 - 1:400 Flow Cytometry: 1.2 ug/10^6 cells CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the CHGA chga for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chromogranin A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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