Rabbit Chromogranin A, CgA Polyclonal Antibody | anti-CgA antibody

Anti-Chromogranin A, CgA antibody

Cat. Num. AAA355098

• Gene Names: CHGA; CGA
• Reactivity: Human, mouse, rat
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: Chromogranin A; CgA; Polyclonal Antibody; Anti-Chromogranin A; CgA antibody; Polyclonal Anti- Chromogranin A; anti-CgA antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid

• Applicable Applications for anti-CgA antibody: Western Blot (WB)
• Application Notes: WB (1:5000-10,000)
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the C-terminal residues of mouse Chromogranin A.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Western Blot (WB)

Related Product Information for anti-CgA antibody

Chromogranin A (CgA), also called parathyroid secretory protein 1, is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A gene has 8 exons and 7 introns spanning about 11 kb1. It is elevated in pheochromocytomas2, and also used as an indicator for pancreas and prostate cancer3 and in carcinoid syndrome4. It also plays a role in early neoplasic progression.

References

1. Wu, H.-J., Rozansky, D. J., Parmer, R. J., Gill, B. M., O'Connor, D. T. Structure and function of the chromogranin A gene: clues to evolution and tissue-specific expression. J. Biol. Chem. 266: 13130-13134, 1991. 2. Cotesta D, Caliumi C, Alò P, Petramala L, Reale MG, Masciangelo R, Signore A, Cianci R, D'Erasmo E, Letizia C (2005). "High plasma levels of human chromogranin A and adrenomedullin in patients with pheochromocytoma". Tumori 91 (1): 53-8. 3. Wu JT, Erickson AJ, Tsao KC, Wu TL, Sun CF (April 2000). "Elevated serum chromogranin A is detectable in patients with carcinomas at advanced disease stages". Ann. Clin. Lab. Sci. 30 (2): 175-8. 4. Nikou GC, Lygidakis NJ, Toubanakis C, Pavlatos S, Tseleni-Balafouta S, Giannatou E, Mallas E, Safioleas M (2005). "Current diagnosis and treatment of gastrointestinal carcinoids in a series of 101 patients: the significance of serum chromogranin-A, somatostatin receptor scintigraphy and somatostatin analogues". Hepatogastroenterology 52 (63): 731-41.

NCBI and Uniprot Product Information

• NCBI GI #: 4502805
• NCBI GeneID: 1113
• NCBI Accession #: NP_001266.1
• NCBI GenBank Nucleotide #: NM_001275.3
• UniProt Accession #: P10645; Q53FA8; Q6NR84; Q96E84; Q96GL7; Q9BQB5; B2R9E9
• Molecular Weight: 50,688 Da
• NCBI Official Full Name: chromogranin-A preproprotein
• NCBI Official Synonym Full Names: chromogranin A (parathyroid secretory protein 1)
• NCBI Official Symbol: CHGA
• NCBI Official Synonym Symbols: CGA
• NCBI Protein Information: chromogranin-A; SP-I; pituitary secretory protein I; parathyroid secretory protein 1; betagranin (N-terminal fragment of chromogranin A)
• UniProt Protein Name: Chromogranin-A
• Protein Family: Glycoprotein hormones
• UniProt Gene Name: CHGA
• UniProt Synonym Gene Names: CgA; SP-I
• UniProt Entry Name: CMGA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.

Subunit structure: Interacts with SCG3

By similarity.

Subcellular location: Cytoplasmic vesicle › secretory vesicle lumen

By similarity. Cytoplasmic vesicle › secretory vesicle membrane

By similarity. Secreted. Note: Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions

By similarity.

Post-translational modification: Sulfated on tyrosine residues and/or contains sulfated glycans.O-glycosylated with core 1 or possibly core 8 glycans. Ref.16 Ref.20

Miscellaneous: Binds calcium with a low-affinity.

Sequence similarities: Belongs to the chromogranin/secretogranin protein family.