Mouse chromogranin A Monoclonal Antibody | anti-CHGA antibody

Mouse Anti-human chromogranin A monoclonal Antibody

Cat. Num. AAA1491959

• Gene Names: CHGA; CGA
• Reactivity: Human. Other species are not tested. Please decide the specificity by homology
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G Purified
• Synonyms: chromogranin A; Monoclonal Antibody; Mouse Anti-human chromogranin A monoclonal Antibody; Chromogranin-A; CgA; Pituitary secretory protein I; SP-I; Vasostatin II; anti-CHGA antibody

• Host: Mouse
• Reactivity: Human. Other species are not tested. Please decide the specificity by homology
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Specific for Recombinant CHGA Protein denatured and native forms
• Purity/Purification: >95%, Protein G Purified
• Sequence Length: 457

• Applicable Applications for anti-CHGA antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant CHGA Protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-47714 / sc-53013 / sc-271738 / sc-376813 / sc-376827
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded Human small intestine tissue using MBS1491959(1D7-3D5) at dilution of 1:200.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded Human pancreas tissue using MBS1491959(1D7-3D5) at dilution of 1:200.)

Western Blot (WB)

(All lanes: Chromogranin-A transfected E.coli lysatelane 1:Mouse Anti--6*his monoclonal antibody at 1ug/mllane 2: Mouse anti- Chromogranin-A monoclonal antibody at 1ug/mlPredicted band size: 52KdObserved band size: 52kd)

Related Product Information for anti-CHGA antibody

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors), and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors. However, CgA is not a tumor-specific antigen for NETs, and an abnormal concentration has been described in some non-malignant diseases such as renal failure, heart failure, proton pump inhibition, gastritis, hypertension, or patients with liver diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 4502805
• NCBI GeneID: 1113
• NCBI Accession #: NP_001266.1
• NCBI GenBank Nucleotide #: NM_001275.3
• UniProt Accession #: P10645; Q53FA8; Q6NR84; Q96E84; Q96GL7; Q9BQB5; B2R9E9
• Molecular Weight: 50,688 Da
• NCBI Official Full Name: chromogranin-A isoform 1 preproprotein
• NCBI Official Synonym Full Names: chromogranin A
• NCBI Official Symbol: CHGA
• NCBI Official Synonym Symbols: CGA
• NCBI Protein Information: chromogranin-A
• UniProt Protein Name: Chromogranin-A
• Protein Family: Chromogranin
• UniProt Gene Name: CHGA
• UniProt Synonym Gene Names: CgA; SP-I
• UniProt Entry Name: CMGA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells.

Protein type: Secreted, signal peptide; Vesicle; Cell adhesion; Secreted

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: extracellular space; mast cell granule; perinuclear region of cytoplasm

Biological Process: defense response to fungus; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; innate immune response; killing of cells of another organism; mast cell activation; mast cell chemotaxis; mast cell cytokine production; mast cell degranulation; negative regulation of catecholamine secretion; negative regulation of vasodilation; organelle organization and biogenesis; ovulation cycle; positive regulation of cAMP metabolic process; regulation of blood pressure; regulation of the force of heart contraction