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Western Blot (WB) (Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-4: A549 cells, 293T cells, Human normal kidney tissue, Hela cells, Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute)

Rabbit anti-Human CHGA Polyclonal Antibody | anti-CHGA antibody

CHGA Antibody

Gene Names
CHGA; CGA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
CHGA; Polyclonal Antibody; CHGA Antibody; CGA; anti-CHGA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total CHGA protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.2 mg/ml (varies by lot)
Sequence Length
457
Applicable Applications for anti-CHGA antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
Immunogen
Synthetic peptide of human CHGA
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-4: A549 cells, 293T cells, Human normal kidney tissue, Hela cells, Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute)

Western Blot (WB) (Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-4: A549 cells, 293T cells, Human normal kidney tissue, Hela cells, Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20,)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20,)
Related Product Information for anti-CHGA antibody
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells.This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system.Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoformshave been found for this gene.
Product Categories/Family for anti-CHGA antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,688 Da
NCBI Official Full Name
chromogranin-A isoform 1 preproprotein
NCBI Official Synonym Full Names
chromogranin A
NCBI Official Symbol
CHGA
NCBI Official Synonym Symbols
CGA
NCBI Protein Information
chromogranin-A
UniProt Protein Name
Chromogranin-A
Protein Family
UniProt Gene Name
CHGA
UniProt Synonym Gene Names
CgA; SP-I
UniProt Entry Name
CMGA_HUMAN

Uniprot Description

CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells.

Protein type: Cell adhesion; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: extracellular space; perinuclear region of cytoplasm; secretory granule

Biological Process: defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; mast cell activation; mast cell chemotaxis; mast cell cytokine production; mast cell degranulation; negative regulation of catecholamine secretion; negative regulation of vasodilation; organelle organization and biogenesis; positive regulation of cAMP metabolic process; regulation of blood pressure; regulation of the force of heart contraction

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Product Notes

The CHGA chga (Catalog #AAA9415918) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHGA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHGA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the CHGA chga for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHGA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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