Rabbit anti-Human CHI3L2 Monoclonal Antibody | anti-CHI3L2 antibody

Recombinant Anti-CHI3L2 Antibody, Rabbit Monoclonal

Cat. Num. AAA8106220

• Gene Names: CHI3L2; CHIL2; YKL39; YKL-39
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A
• Synonyms: CHI3L2; Monoclonal Antibody; Recombinant Anti-CHI3L2 Antibody; Rabbit Monoclonal; CHI3L2/YKL-39 Antibody; Rabbit MAb; chitinase 3 like 2; Anti-CHIL2 Antibody; Anti-YKL-39 Antibody; Anti-YKL39 Antibody; anti-CHI3L2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 213
• Specificity: Human YKL-39/CHI3L2.; No cross-reactivity in ELISA with Human CHIT1, Human CHI3L1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CHI3L2 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:50-1:200
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human YKL-39/CHI3L2 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human YKL-39/CHI3L2 (rh YKL-39/CHI3L2).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CHI3L2 in human lung with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CHI3L2 in human hepatoma with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CHI3L2 in human brain with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-CHI3L2 antibody

Chondrocyte protein 39 (YKL-39), also known as Chitinase 3-like 2 (CHI3L2), is a secretory protein of articular chondrocytes belonging to the glycosyl hydrolase 18 family. Its highest expression is in chondrocytes, followed by synoviocytes, lung and heart. YKL-39/CHI3L2 is not detected in spleen, pancreas, and liver. YKL-39/CHI3L2 may also be expressed in developing brain and placenta. YKL-39/CHI3L2, a cartilage-related protein, is found to induce arthritis accompanied by pathologic changes in bone and cartilage. A better understanding of the immune response against cartilage-related components including YKL-39 may help to elucidate the pathological processes of arthritic disorders. Upregulation of YKL-39/CHI3L2 in osteoarthritic cartilage suggests that YKL-39/CHI3L2 may be a more accurate marker of chondrocyte activation than YKL-40, although it has yet to be established as a suitable marker in synovial fluid and serum. The decreased expression of YKL-40 by osteoarthritic chondrocytes is surprising as increased levels have been reported in rheumatoid and osteoarthritic synovial fluid, where it may derive from activated synovial cells or osteophytic tissue or by increased matrix destruction in the osteoarthritic joint. YKL-39 and YKL-40 are potentially interesting marker molecules for arthritic joint disease because they are abundantly expressed by both normal and osteoarthritic chondrocytes.

References

Sakata M, et al. (2002) YKL-39, a human cartilage-related protein, induces arthritis in mice. Clin Exp Rheumatol. 20(3): 343-50.Areshkov PA, et al. (2010) Chitinase 3-like protein 2 (CHI3L2, YKL-39) activates phosphorylation of extracellular signal-regulated kinases ERK1/ERK2 in human embryonic kidney (HEK293) and human glioblastoma (U87 MG) cells. Tsitol Genet. 44(1): 3-9.Steck E, et al. (2002) Enhanced expression of the human chitinase 3-like 2 gene (YKL-39) but not chitinase 3-like 1 gene (YKL-40) in osteoarthritic cartilage. Biochem Biophys Res Commun. 299(1): 109-15.

NCBI and Uniprot Product Information

• NCBI GI #: 68533255
• NCBI GeneID: 1117
• NCBI Accession #: NP_003991.2
• NCBI GenBank Nucleotide #: NM_004000.2
• UniProt Accession #: Q15782; Q15749; Q15783; Q5VUV7; Q96F97; A6NNY3; B4DPR7
• Molecular Weight: 42,508 Da
• NCBI Official Full Name: chitinase-3-like protein 2 isoform a
• NCBI Official Synonym Full Names: chitinase 3 like 2
• NCBI Official Symbol: CHI3L2
• NCBI Official Synonym Symbols: CHIL2; YKL39; YKL-39
• NCBI Protein Information: chitinase-3-like protein 2
• UniProt Protein Name: Chitinase-3-like protein 2
• Protein Family: Chitinase-3-like protein
• UniProt Gene Name: CHI3L2
• UniProt Entry Name: CH3L2_HUMAN

NCBI Description

The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

CHI3L2: Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity. Belongs to the glycosyl hydrolase 18 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: extracellular space

Molecular Function: chitin binding; chitinase activity

Biological Process: chitin catabolic process