CHI3L2 / YKL-39 recombinant protein

Recombinant Human CHI3L2 / YKL-39 Protein (His tag)

Cat. Num. AAA2545556

• Gene Names: CHI3L2; CHIL2; YKL39; YKL-39
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CHI3L2 / YKL-39; Recombinant Human CHI3L2 / YKL-39 Protein (His tag); YKL-39; YKL39; CHI3L2 / YKL-39 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 390

• Application Notes: The recombinant human CHI3L2 consists of 374 amino acids and has a predicted molecular mass of 42.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCHI3L2 is approximately 43 kDa.
• Predicted N Terminal: Lys 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CHI3L2 / YKL-39 recombinant protein

Background: Chondrocyte protein 39 (YKL-39), also known as Chitinase 3-like 2 (CHI3L2), is a secretory protein of articular chondrocytes belonging to the glycosyl hydrolase 18 family. It highest expression is in chondrocytes, followed by synoviocytes, lung and heart. YKL-39/CHI3L2 is not detected in spleen, pancreas, and liver. YKL-39/CHI3L2 may also be expressed in developing brain and placenta. YKL-39/CHI3L2, a cartilage-related protein, is found to induce arthritis accompanied by pathologic changes in bone and cartilage. A better understanding of the immune response against cartilage-related components including YKL-39 may help to elucidate the pathological processes of arthritic disorders. Up regulation of YKL-39/CHI3L2 in osteoarthritic cartilage suggests that YKL-39/CHI3L2 may be a more accurate marker of chondrocyte activation than YKL-40, although it has yet to be established as a suitable marker in synovial fluid and serum. The decreased expression of YKL-40 by osteoarthritic chondrocytes is surprising as increased levels have been reported in rheumatoid and osteoarthritic synovial fluid, where it may derive from activated synovial cells or osteophytic tissue or by increased matrix destruction in the osteoarthritic joint. YKL-39 and YKL-40 are potentially interesting marker molecules for arthritic joint disease because they are abundantly expressed by both normal and osteoarthritic chondrocytes.

Description: A DNA sequence encoding the human CHI3L2 (NP_003991.2) extracellular domain (Met 1-Leu 390) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 68533255
• NCBI GeneID: 1117
• NCBI Accession #: NP_003991.2
• NCBI GenBank Nucleotide #: NM_004000.2
• UniProt Accession #: Q15782; Q15749; Q15783; Q5VUV7; Q96F97; A6NNY3; B4DPR7
• Molecular Weight: 42,508 Da
• NCBI Official Full Name: chitinase-3-like protein 2 isoform a
• NCBI Official Synonym Full Names: chitinase 3 like 2
• NCBI Official Symbol: CHI3L2
• NCBI Official Synonym Symbols: CHIL2; YKL39; YKL-39
• NCBI Protein Information: chitinase-3-like protein 2
• UniProt Protein Name: Chitinase-3-like protein 2
• UniProt Gene Name: CHI3L2

NCBI Description

The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

CHI3L2: Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity. Belongs to the glycosyl hydrolase 18 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: extracellular space

Molecular Function: chitin binding; chitinase activity

Biological Process: chitin catabolic process