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Western Blot (WB)
(Western blot analysis of SHC1 on human SHC1 recombinant protein using anti-SHC1 antibody at 1/1, 000 dilution.)
Mouse anti-Human, Mouse SHC1 Monoclonal Antibody | anti-SHC1 antibody
SHC1 Antibody
Gene Names
SHC1; SHC; SHCA
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
SHC1; Monoclonal Antibody; SHC1 Antibody; FLJ26504 antibody; p66 antibody; p66SHC antibody; SH2 domain protein C1 antibody; SHC (Src homology 2 domain containing) transforming protein 1 antibody; SHC 1 antibody; SHC A antibody; SHC adaptor protein 1 antibody; Shc antibody; SHC transforming protein 1 antibody; SHC transforming protein antibody; SHC-transforming protein 1 antibody; SHC-transforming protein 3 antibody; SHC-transforming protein A antibody; SHC1 antibody; SHC1_HUMAN antibody; SHCA antibody; Src homology 2 domain-containing-transforming protein C1 antibody; anti-SHC1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
7C-7F
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Sequence Length
583
Applicable Applications for anti-SHC1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of SHC1 on human SHC1 recombinant protein using anti-SHC1 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of SHC1 on human SHC1 recombinant protein using anti-SHC1 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of SHC1 on HEK293 (1) and SHC1-hIgGFc transfected HEK293 (2) cell lysate using anti-SHC1 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of SHC1 on HEK293 (1) and SHC1-hIgGFc transfected HEK293 (2) cell lysate using anti-SHC1 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of SHC1 on NIH/3T3 cell lysate using anti-SHC1 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of SHC1 on NIH/3T3 cell lysate using anti-SHC1 antibody at 1/1, 000 dilution.)
Immunocytochemistry (ICC)
(ICC staining SHC1 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining SHC1 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NIH/3T3 cells with SHC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NIH/3T3 cells with SHC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Related Product Information for anti-SHC1 antibody
Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus activating mitogenic, as well as other, responses. Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate (4-7). Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains.
Product Categories/Family for anti-SHC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
63kDa
NCBI Official Full Name
SHC-transforming protein 1
NCBI Official Synonym Full Names
SHC adaptor protein 1
NCBI Official Symbol
SHC1
NCBI Official Synonym Symbols
SHC; SHCA
NCBI Protein Information
SHC-transforming protein 1
Protein Family
NCBI Description
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Research Articles on SHC1
Similar Products
Product Notes
The SHC1 (Catalog #AAA9435243) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SHC1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SHC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the SHC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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